Comparison of the reactivity of two kinds of (1→3)-β-D-glucan measurement reagents to fungi
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 4
Abstract
Measurements of (1→3)-β-D-glucan are widely used for the diagnosis of deep mycosis, and the amebocyte lysate of a horseshoe crab is used in (1→3)-β-D-glucan reagent. There are two kinds, Tachypleus tridentatus and Limulus polyphemus, used in horseshoe crabs as the materials of a reagent. When the patient plasmas were measured with both reagents that were made of a different horseshoe crab lysate, measurement values did not agree in any samples. Therefore, in order to examine the cause of the discrepancy with both reagents, we compared the reactivity to the fungi released (1→3)-β-D-glucan to 52 strains of 27 species of a deep mycosis. As a result, we confirmed the difference in the reactivity of both reagents in some fungi. It was suggested that the released (1→3)-β-D-glucan from each fungus was not identically, and also that the reactivities of each lysate to (1→3)-β-D-glucan were not different. Those were considered to be one of the causes of the discrepancy with both reagents.
Authors and Affiliations
Tadashi Matsubayashi
Keywords
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