Analysis based on the chemical equilibrium regarding the color development ratio between albumins varying in a straight seed in the dye-binding method
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 4
Abstract
Reaction conditions that make the color development of human serum albumin (HSA) and bovine serum albumin (BSA) equal was analyzed based on their chemical equilibrium. When there are reaction conditions of KPDH<KPDB and nH>nB, or KPDH>KPDB and nH<nB to the equilibrium constant of the color reaction for HSA (KPDH) and BSA (KPDB), and the molar absorptivity ratio (εPD/εD) of the dissociated dye anion (εD) and the dye-protein complex (εPD) for HSA (nH) and BSA (nB), it was estimated that the color development of HSA equals that of BSA depending on the choice of pH, dye and buffer concentrations of the color reagent. In the case of BCG, it was concluded that the relations of KPDH>KPDB and nH<nB exist.
Authors and Affiliations
Yuji Suzuki
Keywords
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