Analysis based on the chemical equilibrium regarding the color development ratio between albumins varying in a straight seed in the dye-binding method
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 4
Abstract
Reaction conditions that make the color development of human serum albumin (HSA) and bovine serum albumin (BSA) equal was analyzed based on their chemical equilibrium. When there are reaction conditions of KPDH<KPDB and nH>nB, or KPDH>KPDB and nH<nB to the equilibrium constant of the color reaction for HSA (KPDH) and BSA (KPDB), and the molar absorptivity ratio (εPD/εD) of the dissociated dye anion (εD) and the dye-protein complex (εPD) for HSA (nH) and BSA (nB), it was estimated that the color development of HSA equals that of BSA depending on the choice of pH, dye and buffer concentrations of the color reagent. In the case of BCG, it was concluded that the relations of KPDH>KPDB and nH<nB exist.
Authors and Affiliations
Yuji Suzuki
Keywords
Related Articles
- EP ID EP140585
- DOI -
- Views 63
- Downloads 0
An international perspective on the derivation of reference intervals
International discussions on reference intervals (RI) date back to the late 1980s when the Expert Panel on a Theory of Reference Values in the IFCC elaborated the concept of reference values and methods for the derivatio...
Stability and reference interval of serum phosphoglucomutase (PGM)
We examined the stability and reference range of serum phosphoglucomutase (PGM) in healthy subjects. Serum obtained from venous blood was stored at -80℃ and 4℃, and the stability of PGM activity was evaluated by measurin...
Recent advances in the examination of B type and C type hepatitis virus
The treatment approach for hepatitis B virus and C virus that are considered to be the main causes of chronic hepatitis, cirrhosis, and liver cancer has been developing into the causal virus treatment in addition to the...
Influence of NaCl on a protein error in the presence of detergent
Influence of NaCl on the color development of protein error in the presence of detergent was investigated using three dyes (BPB, BCG and BCP) and detergents (Brij 35, Triton X-100 and Tween 20). The influence of NaCl on...
Usefulness of Lumipulse Presto Ortho HCV in screening for hepatitis C virus (HCV) infection
Hepatitis C virus (HCV) is a causative agent of liver disease such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HCV is transmitted primarily via blood contact such as the contaminated syringes used in t...