AN IMPROVED RP-HPLC METHOD FOR THE QUANTITATIVE DETERMINATION AND VALIDATION OF LEVETIRACETAM IN BULK AND PHARMACEUTICAL FORMULATION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2015, Vol 6, Issue 8
Abstract
The present study depicts the development of a validated RP-HPLC method for the determination of the Levetiracetam in pharmaceutical tablet dosage form. RP-HPLC method was developed by utilizing Welchrom C18 Column (250 x 4.6 mm, 5µ), Shimadzu LC-20AT Prominence Liquid Chromatograph. The mobile phase composed of 10 mM Phosphate buffer: acetonitrile (50:50 v/v) (pH-3.0, adjusted with triethylamine). The flow rate was set to 1.0 ml/min with the responses measured at 243.2 nm using Shimadzu SPD-20A Prominence UV-Visible detector. The retention time of Levetiracetam was found to be 3.030 minutes. Linearity was established for Levetiracetam in the range of 10-50µg/mlwith correlation coefficient 0.9998. The LOD and the LOQ for Levetiracetam found to be 0.468 μg/ml and 1.421 μg/ml respectively. The amount of Levetiracetam present in the formulation was found to be 99.56 %. None of the excipients interfered with the analyte of interest. Considering all the results of validation parameters simplicity of the method and the cost effectiveness of the overall procedure, it is possible to conclude that the developed method can be suitable for the regular quality control determination of Levetiracetam in bulk as well as pharmaceutical dosage form.
Authors and Affiliations
Ravisankar P, Niharika A, Babu Srinivasa
Keywords
Related Articles
- EP ID EP158935
- DOI 10.7897/2230-8407.068106
- Views 100
- Downloads 0
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF TEMOZOLOMIDE IN PHOSPHATE BUFFER PH 2.0 AS A SOLVENT BY UV SPECTROSCOPY
Temozolomide is an antineoplastic agent with activity against a broad spectrum of murine tumors. This compound is currently marketed for the treatment of patients with glioblastoma multiforme and anaplastic astrocytoma,...
ETHNOPHARMACOLOGICAL POTENTIAL OF CALOTROPIS PROCERA: AN OVERVIEW
The herbal medicine occupy distinct position right from ancient period. The plant Calotropis procera is known by different names in different part of the world. It is a wild growing medicinal plant distributed throughou...
BIODIVERSITY OF FUNGI FROM SILVER ORE WITH REFERENCE TO THE PRODUCTION OF SILVER NANOPARTICLES
Micro organisms take part in soil material transformation process and play an important role in soil formation, fertility evolution, plant nutrient in effect, soil structure formation and improvement and degradation and...
A COMPARATIVE STUDY OF ANTI-CATARACT ACTIVITY OF TRIPHALA AND ITS CONSTITUENTS
Cataract is referred as clouding of lens of the eyes, a major cause of blindness all over the world. There is a wide spread belief that the herbal medicines have fewer side effects compared to allopathic medicines. Hence...
A REVIEW ON AEGLE MARMELOS: A POTENTIAL MEDICINAL TREE
Since time immemorial, plants and their products have been the primary resource of food and medicines for mankind. Aegle marmelos commonly known as bael (or bel), belonging to the family Rutaceae, is a moderate sized, sl...