Abstract

Twenty one different wood decaying wild basidiomycetes fungi were collected from Kolli Hill, out of which five were identified for its ligninolytic potential. In this investigation basidiomycetous fungi were screened the ability to grow on various lignocellulosic substrates (80% moisture) including paddy straw, wood chips, wood powder and wood bark as well grown in solid state fermentation, the maximum fungal growth was noticed in paddy straw. In liquid state fermentation, the maximum growth was observed in basal salt media followed by potato dextrose broth (PDB) and molasses media. The selected basidiomycetes fungi were identified by using nucleotide sequences of the Internal Transcribed Spacer (ITS) region. The phylogenetic tree using maximum likelihood analysis revealed and the same was deposited in Gen bank and accession has been assigned for the same (Accession No. KM596809, KM596810, KM596813, KM596814 and KM596815) and these identified wood decaying fungus were subjected to assayed ligninolytic enzymes like laccase, manganese peroxidase and lignin peroxidase activities in both qualitative and quantitative respectively.

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