Analysis of Abiotic Stress Induced Metabolomic Changes in Vigna radiata
Journal Title: Universal Journal of Environmental Research and Technology - Year 2015, Vol 5, Issue 5
Abstract
The present study involves the use of Phenol, Toluene, Xylene and Hexane as xenobiotics, to induce abiotic stress in mung seedlings under in vitro conditions and to evaluate their impact on seedling’s physiological parameters besides alteration in metabolomic profile with respect to control. The toxicity level was found to be high in case of Phenol followed by Hexane, Xylene and Toluene. Metabolomic analysis revealed that, at low concentration of these compounds, there was an elevation in the contents of the metabolomic parameters such as Total Soluble Sugar, Total Reducing Sugar, Total Phenolic Contents and Total Protein Contents, followed by a sharp decline at higher concentrations. Protein profiling revealed a band of97.4KDaproteins in all the stress induced plant which was not detected in untreated control. This protein is most probably a part of the stress machinery in the plant.
Authors and Affiliations
Gupta Sarika and and De Indranil
Keywords
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