Analysis of ABO serological phenotype and molecular biology results of 256 blood donors with ambiguous blood group
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 7
Abstract
Objective To correctly identify the blood group of ABO and study its molecular biological characteristics. Methods Blood samples from blood donors with discrepancies in forward and reverse typing using the microplate method were subjected to both saline tube agglutination test for serological identification and polymerase chain reaction-sequence specific primers (PCR-SSP) for genotyping. Additionally, direct sequencing of exons 1 to 7 of the ABO gene was performed on some donor samples to analyze their blood phenotype, genotype and gene sequence. Results For 256 samples showing discrepancy between forward and reverse typing in microwell method, 119 were identified as normal ABO blood types, 90 were weakened ABO antibodies and 47 were ABO subtypes by serology tube test. According to the PCR-SSP genotyping test, 233 of 256 (91.02%) were consistent with serological phenotype and genotype, 17 of 256 (6.64%) were inconsistent, and 6 samples can′t read genotype based on the kit result typing table.A total of 17 genotypes were identified in 250 samples as AO1 in 56, AO2 in 58, AA in 50, BO1 in 31, BO2 in 17, BB in 8, O1O1 in 2, O1O2 in 7, AB in 13, AO4 in 1, A205O2 in 1, A205A in 1, A201A in 1, O1O4 in 1, O2O2 in 1, A201B in 1 and A205B in 1. Sequencing of exons 1 to 7 of the ABO gene was carried out in 78 samples, and 29 ABO alleles were detected, seven of which were common alleles (*A101, *A102, *A104, *B101, *O01, *O02, *O04), 22 of which were rare alleles (*A201, *A205, *Ax01, *Ax03, *Ax13, *Ax19, *Ax22, *Ael10, *B305, *Bel03, *Bel06/*Bx02, *Bw07, *Bw12, *Bw17, *Bw37, *O05, *O26, *O61, *B(A)04, *B(A)07, *cisAB01 and*cisAB01var). In addition, six rare allele mutation sites were identified (c.101A>G; c. 103_106delG; c. 146_147insGC; c. 259G>T; c. 322C>T; c. 932T>C). Conclusion The identification of ambiguous ABO blood group requires the combination of serological testing and molecular biological examination to correctly identify the blood type and ensure the safety of clinical blood transfusion.
Authors and Affiliations
Lanlan CHEN, Yanhua ZHANG, Jin CHE, Feng LI, Jingmin MA, Jiahong ZHANG
Keywords
Related Articles
- EP ID EP742811
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.07.008
- Views 11
- Downloads 0
Effect of preoperative anemia on perioperative red blood cell transfusion and prognosis in children with septal defect
Objective To analyze the impact of preoperative anemia on perioperative red blood cell transfusion and prognosis of children undergoing septal defect repair. Methods The medical records of 208 patients under 18 years old...
The in vitro tracing of miR-144/451 reveals the potential regulatory function of LINC01569 in erythropoiesis
Objective Utilizing a specially engineered miR-144-GFP-H1 human embryonic stem cell (hESC) reporter line, this study leverages GFP fluorescence as an indicator of miR-144 expression to gauge the progression of erythropoi...
Evaluation of CLIA plus NAT for detecting HBsAg single-ELISA-reactive samples of blood donors
Objective To study the detection performance of HBsAg single-ELISA-reactive samples of blood donors. Methods Two kinds of ELISA reagents from different manufacturers (named as reagents A and B) were used for HBsAg screen...
Application and evaluation of human antibodies in ABO grouping
Objective To evaluate the application of monoclonal typing reagents and human anti-A/B antibodies for absorption-elution test in ABO grouping. Methods The specificity of monoclonal typing reagents and human anti-A/B anti...
Systematic evaluation and classification of adverse reactions to incompatible blood component transfusion in patients undergoing ABO-incompatible allogeneic hematopoietic stem cell transplantation
Objective To analyze and evaluate the occurrence of adverse reactions to incompatible blood component transfusion in patients undergoing ABO-incompatible allogeneic hematopoietic stem cell transplantation (ABO-incompatib...