Analysis of ABO serological phenotype and molecular biology results of 256 blood donors with ambiguous blood group
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 7
Abstract
Objective To correctly identify the blood group of ABO and study its molecular biological characteristics. Methods Blood samples from blood donors with discrepancies in forward and reverse typing using the microplate method were subjected to both saline tube agglutination test for serological identification and polymerase chain reaction-sequence specific primers (PCR-SSP) for genotyping. Additionally, direct sequencing of exons 1 to 7 of the ABO gene was performed on some donor samples to analyze their blood phenotype, genotype and gene sequence. Results For 256 samples showing discrepancy between forward and reverse typing in microwell method, 119 were identified as normal ABO blood types, 90 were weakened ABO antibodies and 47 were ABO subtypes by serology tube test. According to the PCR-SSP genotyping test, 233 of 256 (91.02%) were consistent with serological phenotype and genotype, 17 of 256 (6.64%) were inconsistent, and 6 samples can′t read genotype based on the kit result typing table.A total of 17 genotypes were identified in 250 samples as AO1 in 56, AO2 in 58, AA in 50, BO1 in 31, BO2 in 17, BB in 8, O1O1 in 2, O1O2 in 7, AB in 13, AO4 in 1, A205O2 in 1, A205A in 1, A201A in 1, O1O4 in 1, O2O2 in 1, A201B in 1 and A205B in 1. Sequencing of exons 1 to 7 of the ABO gene was carried out in 78 samples, and 29 ABO alleles were detected, seven of which were common alleles (*A101, *A102, *A104, *B101, *O01, *O02, *O04), 22 of which were rare alleles (*A201, *A205, *Ax01, *Ax03, *Ax13, *Ax19, *Ax22, *Ael10, *B305, *Bel03, *Bel06/*Bx02, *Bw07, *Bw12, *Bw17, *Bw37, *O05, *O26, *O61, *B(A)04, *B(A)07, *cisAB01 and*cisAB01var). In addition, six rare allele mutation sites were identified (c.101A>G; c. 103_106delG; c. 146_147insGC; c. 259G>T; c. 322C>T; c. 932T>C). Conclusion The identification of ambiguous ABO blood group requires the combination of serological testing and molecular biological examination to correctly identify the blood type and ensure the safety of clinical blood transfusion.
Authors and Affiliations
Lanlan CHEN, Yanhua ZHANG, Jin CHE, Feng LI, Jingmin MA, Jiahong ZHANG
Keywords
Related Articles
- EP ID EP742811
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.07.008
- Views 63
- Downloads 0
Initial screening and retest mode in apheresis platelet donors: a comparative analysis
Objective To investigate the effectiveness of current indicators in initial screening and retest before donation and access the optimal testing strategies. Methods Data of initial screening (rate method for ALT, colloida...
Serological follow-up of anti-SARS-CoV-2 antibodies in blood donors after COVID-19 vaccine booster dose and breakthrough infections in Fuzhou
Objective To analyze the dynamics of specific SARS-CoV-2 IgG antibodies in blood donors in Fuzhou area after receiving booster doses of inactivated COVID-19 vaccine and breakthrough infections, and to provide evidence fo...
Analysis of red blood cell alloimmunization among inpatients: 104 588 cases
Objective To analyze the yield, specificity and detection time of red blood cell(RBC)alloimmunization in 104 588 inpatients. Methods The clinical information of patients who underwent at least one antibody screening in o...
Voluntary blood donation intention of students in middle vocational school in Jinhua and its influencing factors
Objective To explore the intention of voluntary blood donation in students from a middle vocational school in Jinhua and its influencing factors. Methods A total of 400 students were enrolled from a middle vocational sch...
Genotyping identification and sequence analysis of ABO ambiguous blood group in 20 cases
Objective To study the genotypes of ABO ambiguous blood group samples(n=20) and identify their molecular biological characteristics. Methods The serological phenotype of the samples was analyzed by serological techniques...