ANALYSIS OF RENAL EXPRESSION OF TRPM6 AND TRPM7 IN GROWING PIGLETS FED A DIET SUPPLEMENTED WITH INULIN –TYPE FRUCTANS. A PILOT STUDY
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2016, Vol 40, Issue 330
Abstract
A diet enriched with inulin-type fructans has multidirectional, beneficial health effects for both humans and animals. The benefits of such a modified diet include increased intestinal absorption of micro- and macrominerals, including magnesium (Mg2+). It is generally known that Mg2+ is involved in many biological processes, and a disruption of its homeostasis during the growth and development may result in a number of adverse changes. Maintaining the proper balance of Mg2+ involves many mechanisms and factors, among them the recently identified protein TRPM6 and TRPM7 (transient receptor potential melastin 6 and 7). Since the available literature lacks any information about TRPM6 and TRPM7 in farm animals, including swine, we have undertaken this research aimed at identification of these proteins in the kidney of growing piglets and analysis of the impact of diets supplemented with inulin-type fructans on their expressions. The study was performed on 16 male, PIC x Penarlan P76 crossbred piglets. Animals were divided into two groups: the control was fed a standard diet and the treatment group was fed a diet supplemented with 3% aqueous solution of inulin-type fructans. As a result of the study, using Western blotting, we found TRPM6 and TRPM7 in the kidneys of growing piglets. We also found that renal expression of TRPM6 increased in the animals treated with a diet supplemented with inulin-type fructans. Expression of TRPM7, on the other hand, did not change. The increase in TRPM6 expression in the supplement-treated animals presumably contributed to an increased renal retention of Mg2+. The changes in the expression of TRPM6 seem to be a positive effect of the dietary supplementation with inulin-type fructans.
Authors and Affiliations
Katarzyna MICHAŁEK, Weronika MEDEŃSKA, Sandra PIETRZAK, Łukasz STAŚKIEWICZ
Keywords
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