Analysis of the metabolic mechanism in Cantharidin-induced hepatotoxicity in LO2 cells using lipidomics analysis
Journal Title: Toxicology Communications - Year 2021, Vol 3, Issue 2
Abstract
Background: Cantharidin is a major active compound from Banmao (Mylabris). Cantharidin has obvious anticancer activity. However, its clinical application is limited due to serious hepatotoxicity. Methods: To evaluate the toxicity of human liver LO2 cells exposed to cantharidin by lipidomics. After exposing LO2 cells to different doses of cantharidin, the metabolites in LO2 cells were analyzed by nontargeted lipidomics based on liquid chromatography-mass spectrometry. Partial least-squares discriminant analysis and orthogonal partial least-squares discriminant analysis were used to screen differentially expressed metabolites, and then the main metabolic pathways were analyzed. Results: Pattern recognition analysis showed that the lipid metabolite profiles were changed significantly after cantharidin treatment, and 39 differential lipid metabolites were found. Additional analysis showed that these metabolites could mainly involve the metabolic pathways of triglyceride and acylcarnitine for cantharidin toxicity to LO2 cells. Conclusion: Cantharidin has obvious toxic effects on LO2 cells from the perspective of lipid metabolism. Moreover, the LO2 cytotoxicity induced by cantharidin is mainly related to the disorder of triglyceride and acylcarnitine metabolism. It can provide a scientific basis for cantharidin-induced hepatotoxicity.
Authors and Affiliations
Jian-Yong Zhang, Fang Liu, Tian-Mu He, Qi-Yi Wang, Yan Zhang, Xiao-Yan Yuan, Xiao-Fei Li, Can-Can Duan
Keywords
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