Analysis of Vincristine in Leaf and Callus Extract by Validated HPTLC Technique and Its Stress Degradation Studies
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 7, Issue 5
Abstract
Aims: An incipient, concrete, sensitive, selective, precise, and reproducible high-performance thin-layer chromatographic (HPTLC) method was established and validated for estimation of vincristine alkaloid in leaf extract as well as it’s callus. Study Design: Mention the design of the study here. Place and Duration of Study: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India between June 2010 and July 2011. Methodology: HPTLC was performed on aluminium foil precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of 73:25:2, v/v (methanol: toluene: diethyl amine) as a mobile phase. The densitometric analysis was carried out at 254 nm utilizing Camag TLC scanner which engendered compact spots for vincristine (Rf =0.38±0.02). Results: The linear regression data for the calibration plots showed good linear relationship (r2=0.998±0.003) between peak area and concentration in the range of 500-3000 ng spot-1. The method was validated for precision, recovery, robustness, specificity, and detection and quantification limits, in accordance with ICH guidelines. The limits of detection and quantification were 3.06 and 9.29 ng spot-1, respectively. Conclusion: Statistical analysis showed that the method could be prosperously applied for the estimation of vincristine in leaf extract and in callus. As the method could efficaciously separate the drug from its degradation products, it can be employed as a stability indicating one. Moreover, the proposed HPTLC method was utilized to investigate the kinetics of acid degradation process.
Authors and Affiliations
Md. Abul Barkat, Mohd. Mujeeb, Harshita . , Md. Akhlaquer Rahman
Keywords
Related Articles
- EP ID EP344578
- DOI 10.9734/BJPR/2015/18265
- Views 144
- Downloads 0
Anti-angiogenic Potential of Mentha longifolia (Horse Mint): Chlorioallantoic Membrane Assay
Inhibition of angiogenesis is one of the mechanisms of action of anticancer drugs. The current study was designed to probe into anti-angiogenic effect of Mentha longifolia by employing Chorioallantoic membrane assay. Whi...
UV Spectrophotometric Method for the Simultaneous Determination of Desloratidine and Pseudoephedrine HCl in Combined Dosage Form
Aims: UV Spectrophotometric Method for the Simultaneous Determination of Desloratidine and Pseudoephedrine HCl in combined Dosage form. Study Design: A simple, rapid and specific UV spectroscopic method with good sensiti...
Introducing Malaria Rapid Diagnostic Tests (MRDTs) at Registered Retail Pharmacies in Ghana: Practitioners’ Perspective
Aims: In much of Africa, Ghana inclusive, malaria has traditionally been diagnosed and treated presumptively: any patient with fever was presumed to have malaria and treated with antimalarial drugs. In this study, the re...
Antiplasmodial Activity of Leaf Extracts of Zanthoxylum chalybeum Engl.
Background: Medicinal herbs have long been used in the treatment of malaria in the endemic tropical and subtropical regions of the world, especially in sub-Saharan Africa where malaria has remained as a top killer diseas...
Usability of Medicine Package Inserts for Chronic Diseases: A Survey of the Pharmaceutical Market in Jos, Nigeria
Background: The burden of illness is increasingly tending towards chronic diseases while medication adherence is often unsatisfactory. The patient information leaflet is one model that has the prospect to improve medicat...