ANALYTICAL METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF PANTOPRAZOLE AND MOSAPRIDE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
Journal Title: Int J of Pharm Res & Analy - Year 2015, Vol 5, Issue 1
Abstract
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination mosapride and pantoprazole in pharmaceutical dosage form. The column used was BDS in isocratic mode, with mobile phase containing phosphate buffer and acetonitrile (55:45 v/v) adjusted to pH 3.0 with dilute ortho phosphoric acid solution. The flow rate was 1.1 ml/ min and effluents were monitored at 260 nm. The retention times of mosapride and pantoprazole were found to be 2.399 min and 3.191 min, respectively. The linearity for mosapride and pantoprazole were in the range of 7.5-45 μg/ml and 20-120 μg/ml respectively. The recoveries of mosapride and pantoprazole were found to be 99.22 to 100.09% and 98.02 to 99.98%, respectively. The proposed method was validated and successfully applied to the estimation of mosapride and pantoprazole in combined tablet dosage forms.
Authors and Affiliations
Lakshmidurga K*, , Ram Mohan Reddy T , Ajitha A, , Uma MaheswarRao V
Keywords
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