ANALYTICAL METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF PANTOPRAZOLE AND MOSAPRIDE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
Journal Title: Int J of Pharm Res & Analy - Year 2015, Vol 5, Issue 1
Abstract
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination mosapride and pantoprazole in pharmaceutical dosage form. The column used was BDS in isocratic mode, with mobile phase containing phosphate buffer and acetonitrile (55:45 v/v) adjusted to pH 3.0 with dilute ortho phosphoric acid solution. The flow rate was 1.1 ml/ min and effluents were monitored at 260 nm. The retention times of mosapride and pantoprazole were found to be 2.399 min and 3.191 min, respectively. The linearity for mosapride and pantoprazole were in the range of 7.5-45 μg/ml and 20-120 μg/ml respectively. The recoveries of mosapride and pantoprazole were found to be 99.22 to 100.09% and 98.02 to 99.98%, respectively. The proposed method was validated and successfully applied to the estimation of mosapride and pantoprazole in combined tablet dosage forms.
Authors and Affiliations
Lakshmidurga K*, , Ram Mohan Reddy T , Ajitha A, , Uma MaheswarRao V
Keywords
Related Articles
- EP ID EP117508
- DOI -
- Views 136
- Downloads 0
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF RUFINAMIDE IN BULK AND ITS PHARMACEUTICAL DOSAGE FORM
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Rufinamide, in its pure form as well as in tablet dosage form. Chromatography was carried out...
AN OVERVIEW OF ELECTRON PROBE MICROANALYSIS
Electron Probe Microanalysis (EPMA) is an analytical technique that is used to establish the composition of small areas on specimens. It’s one of the several particle-beam techniques These analytical technique has...
RAPID SEPARATION AND ESTIMATION OF LAMIVUDINE AND ZIDOVUDINE IN MARKETED FORMULATIONS USING RP-HPLC METHOD AND ITS VALIDATION
The goal of the present investigation was carried out to develop a validated analytical RP-HPLC method for simultaneous separation and estimation of Lamivudine and Zidovudine for pharmaceutical formulations. The chromat...
ASSESSMENT OF HEAVY METAL CONTAMINATION IN PLASTIC MUGS, FREQUENTLY USED AT LOCAL RESTAURANTS OF DIFFERENT DISTRICTS OF U.P. INDIA
A total number of 42 mugs manufactured from plastics (locally made) were purchased and analyzed to determine the level of zinc, nickel, iron and cadmium. All samples were digested with concentrated nitric acid (HNO...
SUPER CRITICAL FLUID CHROMATOGRAPHY: AN OVERVIEW
Supercritical Fluid Chromatography (SFC) is a form of normal phase chromatography, first used in 1962.SFC typically utilizes carbon dioxide as the mobile phase; therefore the entire chromatographic flow path must be pres...