THREE SIMPLE VALIDATED UV SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF TIMOLOL MALEATE AND BRIMONIDINE TARTRATE AND THEIR COMPARISON USING ANOVA
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 3
Abstract
ABSTRACT Timolol Maleate and Brimonidine Tartrate combination is used for the topical treatment of intra-occular pressure in patients of glaucoma. Three rapid, simple, accurate and precise UV spectrophotometric methods have been developed namely simultaneous equation method (Method 1), First Derivative Method (Method 2) and Ratio First Derivative Method (Method 3) for the simultaneous estimation of Timolol Maleate (TM) and Brimonidine Tartrate (BRT). In Method 1, Timolol Maleate and Brimonidine Tartrate exhibit good linearity over the concentration range of 2-50 μg/ml and 2-14 μg/ml respectively at 294 nm and 255 nm wavelengths. Method 2 is based on First Derivative Method, the ZCP of TM selected for the estimation of BRT is 294.3 nm and the ZCP of BRT selected for the estimation of TM is 302.3 nm. Linearity range is 2-50 μg/ml and 2-14 μg/ml for TM and BRT respectively. Method 3 is based on Ratio First Derivative Spectrophotometry. Linearity range is 2-50 μg/ml and 2-14 μg/ml at 290 nm and 244 nm for TM and BRT respectively. The proposed methods were validated according to the ICH guidelines for the evaluation of accuracy, precision and sensitivity. The three methods were compared using one-way ANOVA and the fcal value was found to be less than ftab value indicating that there is no significant difference in the assay results by the three methods. Thus, the proposed methods are rapid, accurate, precise and economically viable for the simultaneous estimation of TM and BRT respectively in pure form as well as in ophthalmic formulation.
Authors and Affiliations
Heta H. Desai , Anandkumari , D. Captain
Keywords
Related Articles
- EP ID EP126792
- DOI -
- Views 160
- Downloads 0
A REVIEW ON CURRENT TRENDS AND ADVANCEMENTS IN BIOANALYTICAL METHODS
This paper reviews the recent developments in bio-analysis sample preparation techniques and chromatographic techniques give an update on basic principles and a comparative discussion on the benefits and limitation of ev...
SEASONAL WATER QUALITY ASSESSMENT OF SURFACE WATER IN DURG REGION
An investigation was undertaken to determine the Quality of surface water of Shivnath River & ponds in durg and rajnandgaon region”. The study assessed the evolution of water quality in Surface of Durg and Rajn...
RP-HPLC METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF TRITHIOPARAMETHOXYPHENYL PROPANE (ANETHOLE TRITHIONE) AND CHLORPHENIRAMINE MALEATE IN TABLET DOSAGE FORM
ABSTRACT A Simple, precise and rapid RP-HPLC method was developed and validated for simultaneous Estimation of Trithiparamethoxyphenyl propene (Anetholetrithione) and Chlorpheniramine Maleate in tablet dosage form....
DEVELOPMENT OF UV SPECTROSCOPIC METHOD FOR THE DETERMINATION OF GUAIFENESIN IN BULK AND FORMULATION
The present research work discusses the development of UV Spectroscopic method for the estimation of Guaifenesin. Simple, specific, accurate and cost effective spectroscopic method has been developed for the estimation o...
SIMULTANEOUS QUANTIFICATION OF EPIGALLOCATECHIN-3-GALLATE AND ATORVASTATIN BY USING HPLC-UV METHOD AND ITS APPLICATION TO PHARMACOKINETIC STUDIES IN RATS
A sensitive, precise and simple LC method for the simultaneous quantification of epigallocatechin-3-gallate and atorvastatin in rat plasma has been developed and validated. The chromatographic separation was achieved on...