ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF BROMFENAC SODIUM AND MOXIFLOXACIN IN THEIR COMBINED DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 9
Abstract
A simple, rapid, accurate and precise method was developed for Bromfenac sodium and Moxifloxacin. BDS Hypersil C-18 column (250 mm × 4.6 mm id) 5 μm particle size was used as stationary phase. The mobile phase used was Buffer KH2PO4: Acetonitrile : Triethylamine at pH 4.0 adjusted with Ortho phosphoric acid. The mobile phase was delivered at flow rate 1.0 ml/min. UV detection was set at 275 nm. The retention time for Bromfenac sodium and Moxifloxacin was found 4.637 minutes and 7.630 minutes respectively. The linearity was observed over the range of 2.25-6.75 mcg/ml and 12.5-37.5 mcg/ml for Bromfenac sodium and Moxifloxacin respectively. The LOD was found 0.46 mcg/ml and 0.64 mcg/ml for Bromfenac sodium and Moxifloxacin respectively; whereas LOQ was found to be 1.41 mcg/ml for Bromfenac sodium and 1.94 mcg/ml for Moxifloxacin. Moreover, the % RSD for repeatability, Inter and intra-day precision was found to be less than 2 % which reveals method is precise. The correlation co-efficient found to be 0.997 and 0.999 for Bromfenac sodium and Moxifloxacin respectively. The % recovery was found to be 99.79-100.09 % for Bromfenac sodium and 99.90-100.03 % for Moxifloxacin. The assay percentage was found to be 98.65 % and 97.50 % for Bromfenac and Moxifloxacin respectively. All the validation parameters were checked according to ICH guidelines finally it is concluded that the developed method is precise and accurate.
Authors and Affiliations
Prasanna Pradhan , Kuldipsinh Solanki , Umesh Upadhyay
Keywords
Related Articles
- EP ID EP131959
- DOI 10.7897/2230-8407.0509137
- Views 78
- Downloads 0
MOLECULAR MODELING AND DRUG DISCOVERY OF POTENTIAL INHIBITORS FOR ANTICANCER TARGET GENE MELK (MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE)
Maternal embryonic leucine zipper kinase (MELK), a member of the AMP serine/threonine kinase family, exhibits multiple features consistent with the potential utility of this gene as an anticancer target. Reports show tha...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ATENOLOL AND CHLORTHALIDONE FROM PHARMACEUTICAL FORMULATION
A reverse phase high performance liquid chromatography (RP HPLC) method was developed and validated for the simultaneous estimation of atenolol and chlorthalidone in marketed formulation. The determination was carried ou...
A COMPARATIVE PHYTO-PHARMACOGNOSTICAL EVALUATION OF STEM AND ROOT OF SHYONAKA (OROXYLUM INDICUM LINN.)
The root bark of Shyonaka (Oroxylum indicum Linn.), family Bignoniaceae is one of the commonly used drug in Ayurveda. Because of its significant therapeutic activity like anti-inflammatory, analgesic etc its demand is in...
FORMULATION AND EVALUATION OF FAST DISSOLVING TABLETS OF RANITIDINE HYDROCHLORIDE USING DIFFERENT SUPERDISINTEGRANT
Ranitidine HCl is an H2 anhistaminic drug mainly used for treatment of peptic ulcers and is absorbed 50% orally. The drug undergoes hepatic metabolism, so the attempt has been made to administer it as fast dissolving tab...
BIOREACTIVE COMPOUNDS AND ANTIOXIDANT PROPERTIES OF METHANOLIC EXTRACT OF FENNEL (FOENICULUM VULGARE MILLER)
Methanolic extract of dried Fennel (Foeniculum vulgare Miller) seeds was analyzed for the presence of bioreactive compounds: ascorbate, riboflavin, tocopherol and polyphenols with potential antioxidant properties. The ex...