ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF TRIFLUOPERAZINE HCl AND TRIHEXYPHENIDYL HCl IN BULK DRUG AND PHARMACEUTICAL FORMULATIONS
Journal Title: Int J of Pharm Res & Analy - Year 2015, Vol 5, Issue 1
Abstract
RP-HPLC method in which determination of Trifluoperazine hydrochloride and Trihexyphenidyl hydrochloride was carried out by reverse phase C-18 column (Inertsil ODS-3,250*4.6mm) using a mobile phase consisting of acetonitrile: water: triethyelamine (68: 31.8: 0.2v/v) with pH 4 adjusted by using ortho-phosphoric acid. The mobile phase was pumped at rate of 1.0 ml/min and the detection was carried out at 210 nm. The linearity was found in the range of 10-150 μg/ml and 4-60 μg/ml with regression coefficient (r=0.999 for both). The peaks obtained were sharp having clear baselines separation with a retention time of 2.78±5.68 and 2.31 ± 4.72 min for Trifluoperazine hydrochloride and Trihexyphenidyl hydrochloride respectively.
Authors and Affiliations
Shashi Daksh1 , Anju Goyal1 , Chakshu K. Pandiya2
Keywords
Related Articles
- EP ID EP133123
- DOI -
- Views 114
- Downloads 0
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ETHINYL ESTRADIOL AND LEVONORGESTREL
ABSTRACT A simple, accurate, precise, sensitive, specific and reliable stability indicating RP-HPLC method was developed for simultaneous estimation of Ethinyl estradiol (EE) and Levonorgestrel (LEV) in Pharmaceuti...
FORMULATION AND EVALUATION OF ENTERIC COATING TABLETS BY WET GRANULATION METHOD
Serratiopeptidase is derived from bacteria belonging to genus Serratia. Serratiopeptidase tablets used in the treatment of viral diseases and hepatitis. Serratiopeptidase were formulated using HPMC phthalate as e...
RECENT APPROCHES FOR IMPURITY PROFILING IN PHARMACEUTICAL FORMULATIONS
In the pharmaceutical world, an impurity is considered as any other organic material, besides the drug substance, or ingredients, arise out of synthesis or unwanted chemicals that remains with API’s. The impurity m...
EFFECT OF EXCIPIENTS AND PROCESS VARIABLES OVER GASTRO RETENTIVE ANTIHYPERTENSIVE DOSAGE FORM
The objective of this study was to formulate an oral floating tablet of Captopril using the hydrophilic polymer hydroxy propyl methyl cellulose (HPMC) and Carbopol 934P gas generating agent Calcium Carbonate and...
METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF EZETIMIBE AND GLIMEPIRIDE BY RP-HPLC
ABSTRACT A sensitive, selective and precise high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Ezetimibe and Glimepiride in tablet dosage form. T...