ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ESTIMATION METHOD FOR SOTALOL HYDROCHLORIDE TABLETS BY USING RP-HPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 5
Abstract
The present investigation describes about a simple, economic, selective, accurate, precise Reverse phase High performance liquid chromatographic method for the estimation of Sotalol Hydrochloride in pharmaceutical dosage forms. Sotalol Hydrochloride was well eluted using a C18 column of dimension 100 × 4.6, 5µm and Mobile phase consisting of Acetonitrile: 20 mM Ammonium acetate buffer (pH-6): Methanol in the ratio of 10:75:15 v/v/v at the flow rate 1 ml/min and the detection was carried out at 229 nm with UV detector. The Retention time for Sotalol Hydrochloride was found to be 3.314. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Sotalol Hydrochloride pharmaceutical dosage form.
Authors and Affiliations
G Abirami, K Anand Kumar, T. Veterichelvan, Arunateja Muvva
Keywords
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