Anti - Aspergillus flavus Activity of Lactobacillus plantarum and Pediococcus acidilactici Isolated from Breast Milk
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 12
Abstract
The breast milk is not only the best and optimal natural source of essential nutrients for babies, but also provides a broad range of bioactive compounds, as well as its own microbiota, therefore helps to protect body against many diseases too. Lactic acid bacteria (LAB) are the major group of the breast milk microbiota and acts as biocontrol agent in milk. In this study LAB were isolated from the breast milk. Their antifungal activity against aflatoxin producing Aspergillus flavus was investigated with four methods: Study of the LAB isolates influence on biomass production of A. flavus in MRS medium, determination of their inhibitory activity against A. flavus on PDA medium, dual culture overlay assay and finally, effect of LAB cell-free supernatants (CFS) against mycelia growth. The CFS also was analyzed by HPLC and titration experiments. The results indicated that seven isolated LAB had good inhibitory activity against A. flavus growth. Four isolates of LAB were identified as Pediococcus acidilactici and three of them were identified as Lactobacillus plantarum by 16s rDNA sequencing assay. The cell-free supernatants of L. plantarum RS2 expressed strong anti A. flavus activity. Analyzing of their CFS showed that, this strain had significant amounts of propionic acid and hydrogen peroxide in the CFS that might explain the high antifungal activity.
Authors and Affiliations
Sedighi-Khavidak Samaneh| Department of Biology, University of Isfahan, P.O. Box:81746-73441, Isfahan, Iran, Rabbani Khorasgani Mohammad| Department of Biology, University of Isfahan, P.O. Box:81746-73441, Isfahan, Iran, Corresponding Email:m.rabbani@biol.ui.ac.ir, Emtiazi Giti| Department of Biology, University of Isfahan, P.O. Box:81746-73441, Isfahan, Iran, Mazloum-Ardakani Mohammad| Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran
Keywords
Related Articles
- EP ID EP12278
- DOI -
- Views 344
- Downloads 16
The incidence rate of epigastric pain after laryngeal mask anesthesia in cataract surgery: A descriptive-sectional study
Epigastric pain is a condition in which the increase of airway pressure to deal with partial or complete obstruction of airway during mechanical ventilation of lungs occurs or caused by the entrance of too much air to t...
Detection of bacterial pathogens in surgical site infections and their antibiotic sensitivity profile
Surgical site infections considered as a major problem in health care centers, resulting in extended length of stay, substantial associated morbidity and mortality, and high excess hospital cost. Thirty wound swabs were...
PREVALENCE OF CLOSTRIDIUM DIFFICILE TOXIN IN DIARRHOEAL STOOL SAMPLES OF PATIENTS FROM A GENERAL HOSPITAL IN EASTERN PROVINCE, SAUDI ARABIA
Introduction: Clostridium difficile is anaerobic spore- forming bacillus, produces two major toxins (Tcd A and Tcd B). Disease caused by toxigenic C.difficile (Tcd) varies from mild diarrhea to fulminant disease and dea...
RARE PRESENTATION OF RUPTURED RUDIMENTARY HORN PREGNANCY
It is a rare occurrence for the rudimentary horn of uterus to harbour a pregnancy and the usual outcome is devastating leading to a spontaneous rupture in second trimester with the patient presenting in shock with mas...
Identifying the Effects of Education on Burnout in Parents whose Children have Cancer and Presenting Educational-Moral Pattern for Nurses and Nursing Educators in order to Use it in Students' Education
The parents, whose children have cancer, experience a high level of stress and discomfort. The parents' effort in order to adapt to stressful situation depends on the availability and use of supportive sources including...