ANTIBACTERIAL ACTIVITY OF MORINGA OLEIFERA (LAM) LEAVES EXTRACTS AGAINST SOME SELECTED BACTERIA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: The antibacterial activity of Moringa oleifera (Lam.) Leaves extract belonging to the family Moringaceae, was determined using agar well diffusion method against some selected bacteria. Methods: Mueller Hinton Agar (MHA) (Becton Dicknson M. D USA), media was prepared according to the manufacturer’s instruction. Sterile Mueller Hinton agar plates were inoculated with the test culture by surface spreading using sterile wire loops and each bacterium evenly spread on the entire surface of the plate to obtain uniformity of the inoculum. Concentrations of 30, 60, 90 and 120mg/ml prepared from the dry leaves powder were used for antibacterial analysis using agar well incorporation methods. Plates of Mueller hinton agar were prepared and allowed to solidify on Petri dishes. Each plate was then seeded with a test bacterium. Four holes were made in each of the plate with a sterile 2.0 mm diameter cork borers. Each of the four holes was filled with a given concentration of the extract mixed with plane sterile agar. The plates were then incubated at 37°c for 24 hours. The diameters of zones of inhibition were measured using a meter rule and the mean value for each organism was recorded.Results: The aqueous, ethanol and methanol extracts of the plant leaves show an inhibitory effect on the growth of the tested bacteria. For aqueous, ethanol and methanol extracts, the inhibitory effect on Escherichia coli was significantly higher (P<0.05) than that of Staphylococcus aureus and Pseudomonas aeruginosa respectively. In addition, both ethanol and methanol extract showed a significantly higher (P<0.05) inhibitory effect at higher concentration of 120mg/ml.Conclusion: The powder from the leaves of Moringa show potential antibacterial activity against the tested gram positive bacteria; Staphylococcus aureus and gram negative bacteria i. e. Escherichia coli and Pseudomonas aeruginosa.Â
Authors and Affiliations
Kiran Singh, G M Tafida
Keywords
Related Articles
- EP ID EP579530
- DOI -
- Views 66
- Downloads 0
TREATMENT OF TINEA VERSICOLOR CAUSED BY MALASSEZIA FURFUR WITH DILL SEED EXTRACT: AN EXPERIMENTAL STUDY
Objective: Pityriasis versicolor (PV) is a superficial fungal infection of the skin characterized by changes in skin pigment due to colonization of the stratum corneum by a dimorphic lipophilic fungus of the normal flora...
THE EMERGENCE OF TIME PROGRAMMED DRUG DELIVERY SYSTEM: CHRONOTHERAPY OF CARDIO VASCULAR DISEASES
Time programmed drug delivery system is a system that promises to deliver a drug at a point of time when it is most required, as it is programmed beforehand. Though the delivery system is a boon to the pharmaceutical res...
DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PAROXETINE AND CLONAZEPAM IN BULK AND ITS PHARMACEUTICAL FORMULATIONS
A novel stability indicating reversed-phase liquid chromatographic method has been developed and validated for simultaneous estimation of paroxetine and clonazepam in combined pharmaceutical dosage form. An Agilent zorba...
DEVELOPMENT AND VALIDATION OF DIFFERENTIAL PULSE ANODIC STRIPPING VOLTAMMETRIC ANALYSIS OF SELENIUM (IV) IN BULK AND IN DOSAGE FORMULATIONS AT A GOLD ELECTRODE MULTI-MODIFIED WITH A MIXTURE OF 3, 3′-DIAMINOBENZIDINE.4HCL AND VITAMIN E
Objective: A simple, direct and very sensitive differential pulse anodic stripping voltammetric analysis (DPASVA) of selenium (IV) in bulk and in dosage formulations using a gold electrode multi-modified with a mixture o...
PUPAL EMERGENCE INHIBITION ACTIVITY OF ACALYPHA INDICA LEAF EXTRACT AGAINST DENGUE VECTOR, AEDES ALBOPICTUS MOSQUITO
Objective: To investigate the larvicidal activities of six varying extracts of Acalypha indica (A. indica) leaves from family Euphorbiaceae against the dengue mosquito vector, Aedesalbopictus (Ae. albopictus) in laborato...