Antibacterial activity of silver nanoparticles produced by Sesamum indicum seed extract on staphylococcus epidermidis and salmonella typhi
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 6, Issue 5
Abstract
The application of nanoscale materials and structures, usually ranging from 1 to 100 nanometers (nm), is an emerging area of nanoscience and nanotechnology. Nanosilver has been developed as a potent antibacterial, antifungal, anti-viral and anti-inflammatory agent. So far, a plenty of reports have introduced different factors that influence silver nanoparticle toxicity. Such factors are nanoparticle properties like particle size, shape, crystallization, surface chemistry and capping agents. As well as environmental factors like pH, ionic strength, presence of ligands and macromolecular interactions. In this work silver nanoparticles were synthesized using Sesamum indicum seed extracts. Synthesized nano particles were characterized using UV-visible spectrophotometer and Transmission Electron Microscopy (TEM). TEM imageing confirmed the capability of S. indicum seed extract for the synthesis of silver nanoparticles. The antibacterial activity of AgNPs was investigated on Gram-positive bacterium Staphylococcus epidermidis and Gram-negative bacterium Salmonella typhi, through serial dilution method using nutrient broth containing different consentrations of AgNPs. MIC (Minimum inhibitory concentration of growth) and MBC (Minimum bactericidal concentration) were determined.
Authors and Affiliations
Taher mohasseli| Member of Young Researcher Society, M Sc. student in Agricultural Biotechnology, Shahid Bahonar University of Kerman, Iran. email: tahermohasseli3240@yahoo.com, Shahram pourseyedi| Department of Agricultural Biotechnology, Shahid Bahonar University of Kerman, Iran.
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