Antibacterial properties of phytopreparation on Staphylococcus spp
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. The emergence of multiresistant strains of Staphylococci that are difficult to antibiotics and cause severe lesions of soft tissues, sepsis, and complicated surgical pathology are recognized as the one of current infectious diseases problems of animals and humans. One of challenges in pharmacognosy is the search for alternative sources of antibacterial substances with an exhaustive resource of antibiotics of fungal origin. The use of raw medicinal plants is quite promising in this regard. The tendency of scientific research of recent decade reveals a promising range of plants families, which typically contents certain active substances (phytoncides, saponins, alkaloids, glycosides, tannins, essential oils etc.). The goal of the work was to establish the antibacterial effect of plant infusions on reference cryogenic strains of Staphylococcus aureus F-4a (ATCC № 25923) and Staphylococcus epidermidis (14990) in vitro. Materials and methods. Herbal material of 50 species (seeds, grass, shoots, leaves, compound fruit, peel) obtained at different periods of the growing season was used for investigation. The material was classified, dried, and grounded. Samples of 1 g were poured with 5 cm3 of 96% ethanol and were kept it over three weeks in a dry cold place. The obtained alcohol infusion was filtered with sterile multi-layer gauze disc filters. Before the discs were put on the surface of agar with inoculation of the corresponding culture, they were dried in a sterile laminar box under ultraviolet rays. Antibacterial activity of various tinctures was determined by the disk diffusion method in agar with the measurement of the diameter of the growth suppression zone of the culture using a template ruler. Results research and discussion. Concerning the above-mentioned point, we report the results of the use of tinctures in Staphylococcus aureus, Staphylococcus epidermidis in vitro. Obtained data has been systematized, summarized and evaluated. Conclusions and perspectives of further research. It has been determined that tinctures of Eleutherococcus senticosus, Eleutherococcus sessiliflorus and Punica granatum show the antibacterial effect on cryogenic reference strains of Staphylococcus aureus F-4a (ATCC №25923), as well as Punica granatum and propolis show the antibacterial effect on cryogenic reference strains of Staphylococcus epidermidis (14990), so the tinctures involved can be recommended to combat multiresistant strains of Staphylococcus.
Authors and Affiliations
Volodymyr Zazharskiy, Pavel Davydenko, Oleg Kulishenko, I. V. Borovik
Keywords
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- EP ID EP389343
- DOI 10.31073/vet_biotech32(2)-21
- Views 94
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