Antibiotics Susceptibility Profile of Listeria Species Isolated from Poultry Wastes and Fishpond Water from Private and Institutional Farms in Ibadan, Nigeria
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 13, Issue 4
Abstract
Introduction: Untreated waste being discharged into the environment due to proliferation of poultry and fish farms can constitute a public health threat to human. Listeria, an emerging pathogen is commonly associated with food. This study aimed at determining the antibiotic resistant pattern of Listeria species isolated from poultry droppings and fish pond water in Ibadan. Materials and Methods: Poultry waste and fishpond water samples were collected between April and July, 2016. Listeria Selective Agar was used to isolate Listeria species and identified using conventional methods. Antimicrobial susceptibility testing was done using the Kirby-Bauer disk diffusion method against ampicillin, cloxacillin, amoxicillin, streptomycin, ceftriazone, chloramphenicol, ciprofloxacin, ofloxacin, sulfamethoxazole-trimethoprim and tetracycline. Results: A total of forty samples were collected and 105 Listeria spp. isolated; 62.9% from poultry waste and 37.1% from fishpond water. From the institutional fish pond and poultry waste, 18.1% and 51.4% isolates were obtained while from private fish pond and poultry waste, 19.1% and 11.4% isolates respectively were obtained. The isolates were Listeria monocytogenes (27.6%), L. innocua (8.6%), L. ivanovii (16.2%) and other Listeria spp. (47.6%). All the isolates were resistant to ampicillin and ciprofloxacin; all L. monocytogenes, L. innocua and L. ivanovii were resistant to ceftriaxone, but 89.3% of the other Listeria spp. showed resistance. Furthermore, all (100%) the isolates obtained from private poultry farm were resistant to chloramphenicol while 53.3% were resistant to trimethoprim/sulfamethoxazole. Also, 12.2% of the isolates were found to be resistant to a combination of six antibiotics including: Ampicillin, amoxicillin, ceftriaxone, streptomycin and trimethoprim/sulfamethoxazole. Conclusion: This study revealed that poultry waste and fish pond water from the selected farms could be a potential source for the transmission of multi-drug resistant bacteria to humans. Proper treatment of poultry waste and fish pond water should be ensured before discharge into the environment.
Authors and Affiliations
Olutayo Israel Falodun, Moturayo Janet Amusan
Keywords
Related Articles
- EP ID EP318185
- DOI 10.9734/JABB/2017/34566
- Views 48
- Downloads 0
Effects of Some Cultural Conditions on Keratinase Production by Bacillus licheniformis Strain NBRC 14206
Aims: Microbial keratinase is an extracellular enzyme capable of degrading keratin present in feathers, hair, and wool. They are widely used in chemical, medical industries as well as in animal feed industry and basic bi...
Inhibition of Biogenic Sulphide Production and Biocorrosion of Carbon Steel by Sulphate-reducing Bacteria Using Ocimum gratissimum Essential Oil
The inhibition effects of Ocimum gratissimum essential oil (OGEO) on biogenic sulphide production and biocorrosion of carbon steel by Sulphate-reducing bacteria (SRB) were investigated in the laboratory. SRB were isolate...
Effect of Climate in Seed Diversity of Wild Tunisian Rhus tripartita (Ucria) Grande
Rhus tripartita (Ucria) Grande (Anacardiaceae) is a rare and endangered species. Seeds of Rhus tripartita were obtained from spontaneous populations in nine different localizations in Tunisia and their shape analyzed by...
The Use of Plant Dyes for Microbial Staining and Identification: An Eco-friendly and Non-Toxic Alternative Method
Staining of microbial cells is a very important procedure in microbial identification. Cells need to be fixed and stained to increase visibility, accentuate morphological features and for preservation. The study was carr...
Evaluation of Bacillus thuringiensis Snkr10 as a Potent Plant Growth Promoting Phyllospheric Bacterium Isolated from Spinach (Spinacia oleracea) Plants
The phyllospheric microorganism used leaf surface of plants as habitat and help to maintain the plants growth by behaving as plant growth promoting bacteria (PGPB). SNKr10 is a gram positive and rod shaped spinach phyllo...