ANTICANCER POTENTIAL OF METHANOLIC AND AQUEOUS EXTRACT OF LEUCAS INDICA (LINN.) AGAINST EHRLICH ASCITES CARCINOMA CELLS ON SWISS ALBINO MICE
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
The present study was designed to evaluate the in vivo anticancer activity of methanolic and aqueous extract of Leucas indica Linn. (Aerial parts) against Ehrlich’s Ascites Carcinoma (EAC) on Swiss albino mice. The anticancer activity was done by intra peritoneal transplantation of EAC cells into mice and subsequently analysis of ascitic fluid, haematological, biochemical and histopathological parameters along with body weight and percentage of life span. The animals were treated with the extracts at the dose level of 200 and 400 mg/kg, intra peritoneally and compared to the reference drug vinblastine. Both the extracts showed significant dose dependent reduction of viable cells (cancer cells), increased non-viable cells (dead cells), decreased in body weight and increased the duration of life span. In case of hematological study the extracts showed increased hemoglobin level and RBC count but decreased WBC count. The certain important serum enzymes like glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and alkaline phosphatase levels were also decreased in treated group of animals. Finally, in case of histopathological investigation of livers showed a significant protection and regeneration of EAC induced oxidative stress and cellular damage. The preliminary phytochemical screening of the extracts revealed the presence of flavonoids, total phenolic compounds, saponin and tannin. However the aqueous fraction of the Leucas indica possesses more potential anticancer property compared to methanolic fraction. The anticancer property of this plant was considered due to the presence of flavonoids and total phenolic compounds.
Authors and Affiliations
Mahananda Sarkar , Mousumi Das , Debmalya Mitra , Aditya Jena , Arnab De , Amalesh Samanta
Keywords
Related Articles
- EP ID EP151503
- DOI 10.7897/2230-8407.04641
- Views 95
- Downloads 0
FORMULATION AND EVALUATION OF LORNOXICAM FAST DISSOLVING TABLET
The goal of the present investigation was to design and evaluated taste mask oral disintegration tablet of lornoxicam, which is NSAID by sublimation & effervescent method using various excipients (menthol, camphor, c...
FORMULATION AND EVALUATION OF POSITIVELY CHARGED SELF-EMULSIFYING DRUG DELIVERY SYSTEM CONTAINING IBUPROFEN
One of the most persistent problems faced by the formulation scientists has been to find methods of improving the oral bioavailability of poorly water-soluble drugs. This positively charged SEDDS gives several fold incre...
HPTLC DETERMINATION OF SORAFENIB TOSYLATE IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORM
A simple and sensitive HPTLC method has been developed for the quantitative estimation of Sorafenib Tosylate in bulk and Pharmaceutical dosage form. Sorafenib Tosylate was chromatographed on silica Gel 60 F254 TLC plate...
METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF FLUPIRTINE MALEATE IN BULK AND PHARMACEUTICAL DOSAGE FORMS USING UV-VIS SPECTROPHOTOMETRY
The present study describes a simple, accurate, precise and cost effective UV-VIS Spectrophotometric method for the estimation of Flupirtine an analgesic drug in bulk and pharmaceutical dosage form. The solvent used is m...
SYNTHESIS OF 6, 7-DISUBSTITUTED-2-ARYL-4H-1-BENZOPYRAN-4-ONES AS ANTIBACTERIAL AGENTS
Benzopyrones constitute one of the major classes of natural products. Both semisynthetic and natural benzopyrone derivatives are known to possess important biological properties and as a result several attempts and proce...