Anticancerous Potentiality of in vivo Grown Twigs and in vitro Generated Plantlets of Endangered Oroxylum indicum (L.) Vent.
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 2
Abstract
The pharmacological and cytotoxicity evaluation of natural products, extracted from endangered medicinal plants have been reported in several cases. In the present study an attempt was made to evaluate the ethanol extract of both in vivo grown twigs and in vitro generated plantlets of Oroxylum indicum (L.)Vent. with respect to cytotoxicity against human cervical cancer SiHa cells and liver hepatocellular HepG2 cells. MTT assay measured the level of cytotoxicity of ethanol extract of both samples of in vivo and in vitro sources. In later case, plant tissue culture techniques were employed to raise in vitro generated plantlets, for which the nodal regions of in vitro seedling were taken as explants. MS medium, supplemented with BAP (2.5 µg ml-1 ) proved better than 2,4-D for shoot initiation. Such differentiated shoots on transfer into half strength MS media with IBA (0.5 µg ml-1 ) resulted into rooted plantlets and were exposed for ethanol extraction after 35 days incubation in acclimatizer room. The dose dependent cytotoxic effect of ethanol extract of in vivo sample in case of SiHa and HepG2 cells on extrapolation resulted into IC50 at 440 and 480 µg ml-1 , respectively whereas in case of in vitro samples, the IC50 for SiHa and HapG2 cells was deducted at 530 and 550 µg ml-1 , respectively. Thus, it was confirmed that the cytotoxic effect of natural products of O. indicum was more vigorous by 15% in field plants as compared to tissue culture generated plantlets and such difference could be possibly attributed to the changed habitat. The effect of extract on dying cells was further confirmed when apoptosis was measured by DNA fragmentation on agarose gel.
Authors and Affiliations
Nishi Kumari, Rajeev Kumar, Kanak Sinha, Santosh Kumar
Keywords
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- EP ID EP415079
- DOI 10.9790/264X-0402022127.
- Views 181
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