Antifungal Susceptibility Testing of Dermatophytes
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 12
Abstract
Abstract: There is an increased prevalence of dermatophytes over the past few decades. Although recent antifungals have a high success rate, treatment failure may occur in 20% cases due to antifungal drug resistance. This study was done to determine antifungal susceptibility of identified dermatophytes to azole group of antifungal drugs. A total of 100 skin, hair and nail samples from clinically suspected cases of dermatophytosis were cultured on Sabouraud dextrose agar and Dermatophyte test media. Positive cases were further processed by subculturing on Potato Dextrose Agar and antifungal susceptibility against 3 azoles, fluconazole, itraconazole and ketoconazole was done by microbroth dilution method according to CLSI guidelines M38A2.Out of 100 clinically suspected cases of Dermatophytosis, Mean MIC (Minimum inhibitory concentration) of Fluconazole was 16.08µg/ml, Itraconazole was 0.239µg/ml and Ketoconazole was 0.339µg/ml. To conclude, in this study, Itraconazole with lowest MIC was most sensitive and most potent against dermatophytes Fluconazole with highest MIC was least sensitive and most resistant. Ketoconazole had intermediate MIC and sensitivity. Keywords:Dermatophytes, Azole Antifungals, Resistance, Microbroth dilution, CSLI, MIC.
Authors and Affiliations
JK Surekha, Chandana Konda, D. Sudha Madhuri, K. Nagamani
Keywords
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