ANTIMICROBIAL AND PHYTOCHEMICAL ANALYSIS OF ALOE VERA L
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 10
Abstract
The present study was made to attempt the antimicrobial and phytochemical analysis of Aloe vera L (babosa). The antimicrobial activity method was using Muller and Hinton agar Dimethyl sulfoxide (DMSO) was used. The Overnight incubated bacterial culture, Staphylococcus aureus, Bacillus subtillis, Proteus vulgaris, Pseudomonas aeroginosa, Enterobacter aerogenes, Klebshiella sp, Salmonella sp, Shigella sonie, S. spidermiods. In phytochemical studies, the leaf extract were analysed for the flavonoids, pholabatannis, glycosides, phenols, catachol, resins, saponins, lipids and fats, tannis, acidic compounds, terpenoids, reducing sugars, anthraquinone, carbohydrates, steroids, and sterols etc. In analysis of Tannin compounds brownish green colour developed to indicate the presence of Tannin. In this screening process Tannin, Saponin, Flavonoids and Terpenoids compounds revealed positive results Antibacterial activity of A.vera was analysed against E.coli, Enterobacter aerogens, Staphylococcus sp, Proteus mirabilus, Pseudomonas sp., Shigella sonie, Salmonella sp, S. spidermiods, Klebshiella sp. Among the three bacterial organisms maximum growth suppression was observed in Staphylococcus sp, Enterobacter aerogens and Klebsiella sp. Anti bacterial activity of A.vera was analysed against Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and E. coli. A.vera leaf gel can inhibit the growth of two gram positive bacteria Shigella flexneri and Streptococcus pyogenes. Specific plant compounds such as anthroquinones and dihydroxy anthroquinones as well as Saponins have been proposed to have direct antimicrobial activity. The antioxidant activity of A.vera leaf and gel extract by using DPPH free radical scavenging assay method. The antioxyactivity of A.vera leaf and gel aqueous extract was determined at the concentration (100, 200, 300, 400 and 500 µg/ml) and IC 50 was calculated. In, DNA protective activity of A.vera on blood DNA against free radicals generated by H2O2. The results indicator that plant leaf extract had maximum DNA protective activity than gel extract against free radicals. The present study A.vera gel and leaf extract was analysed by HPLC chromatogram and should the presence of glucomannose, galactoglucoaralimannone and gluconic acid, vitamin C and also anthraquinone, phenols, and chromones.
Authors and Affiliations
V Mariappan , G Shanthi
Keywords
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