ANTIMICROBIAL POTENTIAL OF FUNGAL ENDOPHYTES ISOLATED FROM LEAVES OF PROSOPIS JULIFLORA (SW.) DC. AN IMPORTANT WEED
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 12
Abstract
Objective: To evaluate the antimicrobial potential of fungal endophytes isolated from leaves of Prosopis juliflora against the important plant and human pathogenic bacteria and fungi.Methods: Antimicrobial screening was done by agar diffusion and dual culture method. The ethyl acetate extract of the endophytes which recorded good activity were subjected to disc diffusion assay and determination of MIC.Results: A total of 446 fungal isolates were recovered from 400 leaflet segments representing 44 fungal endophytes. Species of Cladosporium, Colletotrichum and Fusarium were the dominant genera. Seventeen endophytic fungi out of 44 showed significant antibacterial activity against one or more test bacteria. Ethyl acetate extract of Colletotrichum gloeosporioides exhibited highly significant broad spectrum antibacterial activity. Eleven endophytes showed antifungal activity against all test fungi. Paecilomyces lilacinus and Trichoderma sp. showed significant antifungal activity with growth inhibition of 50% and above in dual culture. The ethyl acetate extract of Paecilomyces lilacinus also showed significant antifungal activity in disc diffusion. This is the first report of antimicrobial potential of the endophyte Paecilomyces lilacinus. Preliminary biochemical characterization of the active ethyl acetate extract of Colletotrichum gloeosporioides and Paecilomyces lilacinus showed the presence of alkaloids, carbohydrates, sterols and coumarins.Conclusion: Results of the study paves the way for further studies on the isolation and characterization of antimicrobial principles and their evaluation for the agricultural and medical application.Â
Authors and Affiliations
Aparna Srivastava, Raveesha Koteshwar Anandrao
Keywords
Related Articles
- EP ID EP578045
- DOI -
- Views 94
- Downloads 0
IDENTIFICATION OF SILDENAFIL CITRATE AS AN ADULTERANT IN HERBAL PRODUCTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH PHOTODIODE ARRAY DETECTOR
Objective: Investigation of sildenafil citrate as an adulterant in the traditional liquid herbal products of the local market in Bangladesh.Methods: A reversed-phase high-performance liquid chromatographic (HPLC) method...
AWARENESS ON CERVICAL CANCER-RISK ASSESSMENT
Objective: To create awareness on cervical cancer, a level of knowledge about cervical cancer, to be advised of effective screening procedures based on their risk assessment, to educate about preventive measures after sc...
OVERCOMING CHEMORESISTANCE IN NON-HODGKIN LYMPHOMA PRELIMINARY STUDIES OF APOPTOSIS AND NECROSIS BY P-GLYCOPROTEIN REVERSAL AGENTS
Objective: The in vitro measurement of drug-induced apoptosis provides a mechanism-based test for the chemosensitivity of tumor cells isolated from a patient or from a specific cell line. The goal of this study was to in...
DESIGN AND STATISTICAL OPTIMIZATION OF ANTACID ANALGESIC EFFERVESCENT TABLETS BY USING 2^3 FACTORIAL DESIGN
Objective: The present research work was undertaken with an aim to formulate a combination dosage form constituting an antacid and analgesic which may be useful in case of hyperacidity associated headache.Methods: In thi...
GANGRENE PRECIPITATED BY CISPLATIN BASED CONCURRENT CHEMORADIATION IN CANCER CERVIX
Gangrene of hand leading to its amputation may be the worst after-effects of cisplatin. Here we report the case of cisplatin-induced gangrene in a patient of the cancer cervix who was treated with concurrent chemo radiat...