ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXIC ACTIVITIES OF VARIOUS EXTRACTS OF THYSANOLAENA MAXIMA (ROXB) KUNTZE AVAILABLE IN CHITTAGONG HILL TRACTS OF BANGLADESH
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: To evaluate the presence of different phytoconstituents and investigate in vitro bioactivities of petroleum ether, chloroform and methanol extracts of Thysanolaena maxima available in Bangladesh.Methods: Phytochemical screening was conducted using the specific standard procedure. Antioxidant activity of the extracts was evaluated using DPPH radical scavenging assay and reducing power assay. Determination of total phenolic and flavonoid contents was also carried out. Antibacterial and cytotoxic activities were investigated using disc diffusion method and brine shrimp lethality bioassay, respectively.Results: The methanol extract showed highest DPPH radical scavenging activity as well as possessed highest phenolic content (IC50 value for DPPH is 36.94±0.62 μg/ml and total phenolic content is 74.39±2.87 in mg/g, GAE) compared to the petroleum ether and chloroform extracts. On the other hand, chloroform extract possessed maximum flavonoid content (81±7.542 in mg/g, QE) and highest reducing power compare to other extracts. All the extracts showed mild to moderate in vitro antibacterial activity with a zone of inhibition ranging from 7 mm to 16 mm. In brine shrimp lethality bioassay, the LC50 values for petroleum ether, chloroform and methanol extracts were found to be 579.05±78.08 μg/ml, 386.92±80.47 μg/ml and 494.29±104.82 μg/ml, respectively which revealed weak cytotoxic potentials of the extracts compared to the positive control.Conclusion: The results indicated that T. maxima could be a very potent source of natural radical scavenger. Isolation of active compounds from this plant responsible for producing such bioactivities is underway.Keywords: Thysanolaena maxima, DPPH, Total phenolic contents, Total flavonoid contents, Reducing power, Cytotoxicity, Antimicrobial activity
Authors and Affiliations
Nazia Hoque, Md. Hossain Sohrab, Trisha Debnath, Md. Sohel Rana
Keywords
Related Articles
- EP ID EP576231
- DOI -
- Views 60
- Downloads 0
LARVICIDE AND ANTIFUNGAL ACTIVITIES OF SARSAPARILLA (SMILAX LARVATA) EXTRACTS
Objective: This study aimed to investigate the toxicity, antimicrobial and larvicide activities of Smilax larvata Griseb. (Smilacaceae) extracts.Methods: Toxicity evaluation was performed by testing brine shrimp lethalit...
CHOLESTATIC LIVER FIBROSIS IN A RAT MODEL OF BILE DUCT LIGATION: EVALUATING BIOCHEMICAL VERSUS HISTOPATHOLOGICAL CHANGES
Objective: Bile duct ligation (BDL), chronic liver injury model, was extensively used in studying mechanisms of fibrogenesis and antifibrotic agents. Considering the liver regenerative capacity and the diverse results fr...
DECIPHERING THE ACTION MECHANISM OF INDONESIA HERBAL DECOCTION IN THE TREATMENT OF TYPE II DIABETES USING A NETWORK PHARMACOLOGY APPROACH
Objective: The aim of this research was to investigate action mechanism of Indonesia herbal decoctions in the treatment of Type 2 Diabetes (T2D) using network pharmacology approaches.Methods: Drug target profile analysis...
STUDY ON THE PROPERTIES OF PURIFIED RECOMBINANT SUPEROXIDE DISMUTASE FROM STAPHYLOCOCCUS EQUORUM, A LOCAL ISOLATE FROM INDONESIA
Objective: Superoxide dismutase (SOD) (E. C: 1.15.1.1) from Staphylococcus equorum which catalyzes the dismutation of the superoxide anion (O2.-) into molecular oxygen (O2) and hydrogen peroxide (H2O2), is one of the mos...
UTILIZATION OF ALCOHOLIC BEVERAGE DISTILLERAY WASTE WATER FOR PRODUCTION OF DOCOSAHEXAENOIC ACID
Objective: The marine thaustochytrid Schizochytrium sp known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications. In the present study, distillery waste was studi...