Application of capillary electrophoresis using discontinuous buffer system in bovine serum albumin analysis
Journal Title: Science Paper Online - Year 2007, Vol 2, Issue 12
Abstract
In this study a capillary electrophoresis (CE) application technique uses different buffers in the equilibration process (equilibrating buffer) and the electrophoresis process (working buffer) is developed. When low concentration phosphate buffer is used to equilibrate and high concentration buffer as working buffer, the sample is stacked effectively. Contrarily the separation of samples bovine serum albumin (BSA) is enhanced when high concentration phosphate buffer is used as the equilibrating buffer. The addition of methanol in the working buffer effectively improves the separation of BSA.
Authors and Affiliations
Xiaonan LIU, Xian-ai SHI, Hang WANG
Keywords
Related Articles
- EP ID EP107651
- DOI -
- Views 103
- Downloads 0
沥青基球状活性炭对若干酶类分子的吸附性能研究
考察具有不同比表面积及不同孔结构的沥青基球状活性炭(PSAC)对溶液中有益分子-淀粉酶和胃蛋白酶的吸附行为，并对其孔结构的相关性进行关联。结果表明：PSAC对-淀粉酶及脂肪酶选择性差，对体内有益分子的吸附量比药用炭少的多。几乎全部...
Effect of aeration on characteristics of the activated sludge and membrane fouling in a submerged membrane bioreactor
Laboratory experiments on submerged MBR were carried out to investigate the influence of aeration on characteristics of the activated sludge and membrane fouling. Results showed that membrane fouling included 2 stages un...
剪切盒加载下岩石断裂特征的有限元分析
本文介绍Ⅱ型断裂的研究现状，指出对于脆性岩石，并非Ⅱ型加载必定会产生对应的Ⅱ型断裂，借鉴剪切盒加载限制岩石发生Ⅰ型断裂，从而实现真正意义上的Ⅱ型断裂，并利用有限元方法分析在Ⅱ型加载下岩石裂纹尖端的应力场，探讨了岩石在Ⅱ型加载下...
无线传感器网络分簇路由改进算法及其性能
针对分簇路由和多跳传输存在的不足，提出一种无线传感器网络(WSNs)分簇路由改进算法，并进行性能分析。改进算法的簇首选择基于节点的剩余能量，簇的建立时，节点综合考虑了节点到簇首及簇首到基站的复合距离，选择复合距离最小的簇加入；...
Study on axon outgrowth of PC-12 cell affected by polypyrrole
Three polypyrrole films are electrodeposited on glass substrate by changing the dopants and the numbers of films. Scanning electron microscopy (Hitachi-530 SEM) is used to characterize surface morphology. The numbers of...