Application value of nucleic acid testing in syphilis screening reactive blood
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 2
Abstract
Objective To evaluate the application value of nucleic acid testing (NAT) by studying the NAT-yield of syphilis screening reactive blood from five blood centers. Methods The blood samples and demographic information of syphilis screening positive donors were collected from five domestic blood centers, i. e. Chongqing, Guangxi, Luoyang, Liuzhou, Mianyang and Urumqi. The treponema pallidum particle agglutination (TPPA) and the established SYBR Green qPCR method were used to analyze the difference between the results of NAT and the other two test results. Results Among 1 679 reactive blood samples for syphilis screening, 819 were confirmed positive by TPPA, accounting for 49%, with the false positive rate exceeded 50%. As to NAT results, the NAT-yield of syphilis screening reactive samples and confirmed positive samples was the same (both 2.20%); the NAT-yield of TPPA-positive and TPPA-negative samples were 2.20% and 2.74%, respectively. Conclusion Primary syphilis screening by ELISA has high sensitivity, but also presents high false positive rate. Although TPPA confirmatory test has strong specificity, it cannot reflect the existence of T. pallidum. Therefore, NAT may be used as a supplementary test for syphilis screening so as to more effectively ensure the safety of blood transfusion and blood supply.
Authors and Affiliations
Anqing LIU, Qiaoling ZHOU, Miao HE, Zhan GAO
Keywords
Related Articles
- EP ID EP738490
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.02.016
- Views 46
- Downloads 0
Preliminary analysis of qualitative changes in suspended red blood cells under different low-pressure and hypoxic conditions
Objective To evaluate the quality of suspended red blood cells (SRBC) under low-pressure and hypoxic conditions, in order to lay a theoretical foundation for airline delivery, air drop and storage of SRBC on plateau. Met...
Retest improvement for minipool nucleic acid testing positive samples
Objective To analyze the correlation between the distribution interval of minipool nucleic acid testing(NAT) positive CT value and the resolution rate, so as to improve the retest model and reduce residual risk of blood...
Removal of serological interference in the treatment of multiple myeloma with CD38 monoclonal antibody using polybrene method
Objective To investigate the clinical feasibility of polybrene method to remove serological interference in patients receiving anti-CD38 monoclonal antibody for the treatment of multiple myeloma, and its detection perfor...
Effect of online and offline collaborative and integrated mode on voluntary blood donation: Internet + blood donation souvenir
Objective To explore the effect of online and offline collaborative and integrated mode (Internet + blood donation souvenirs), and develop diversified scientific ways of caring for voluntary blood donors in combination w...
Expression of platelet microparticles in patients with diabetic nephropathy and its relationship with renal damage
Objective To investigate the expression levels of platelet microparticles(PMPs) in patients with diabetic nephropathy (DN) and their relationship with renal injury. Methods Thirty DN patients, 30 T2DM patients without DN...