AQBD ASSISTED SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF CEFIXIME TRIHYDRATE USING TWO OXIDATIVE COUPLING AGENTS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 7
Abstract
Objective: The present study focuses on developing two visible spectrophotometric methods for cefixime trihydrate (CFT) by the utilization of analytical quality by design space concept. The critical method variables were screened and optimized by factorial design. Methods: In methods A and B, CFT reacts with 3-methyl-2-benzothiazolinone hydrazone hydrochloride in an acidic medium having λmax at 630 nm and CFT reacts with 1, 10 phenanthroline (Phen) showing λmax at 510 nm, respectively. Fractional factorial design was employed for the initial screening of independent variables for both methods. The main, interaction, and quadratic effects of the most significant factors on the preferred response (absorbance) for methods A and B were studied employing central composite design (CCD) with response surface methodology. Results: The method was linear (r2=0.9983 and 0.9986 for methods A and B), accurate (mean recovery = 100.06% and 99.26% for methods A and B), precise (relative standard deviation, % relative standard deviation = 0.9214, 1.025 and 0.1919, 0.2136 for methods A and B). Conclusion: The outcome of the proposed work has evidently revealed that quality by design concept can be efficiently enforced in the optimization of spectrophotometric technique for the quantification of CFT in pharmaceutical raw materials and formulations by minimum experimental runs.
Authors and Affiliations
KOKILAMBIGAI K S , LAKSHMI K S
Keywords
Related Articles
- EP ID EP635669
- DOI -
- Views 112
- Downloads 0
DECREASEMENT OF LYSOPHOSPHATIDYLCHOLINE LEVEL, NF-KB EXPRESSION, INTIMA MEDIA THICKNESS AND IMPROVEMENT OF INSULIN RESISTANCE BY DARAPLADIB TREATMENT: IN VIVO STUDIES OF TYPE 2 DIABETES MELLITUS SPRAGUE-DAWLEY RAT MODEL
Objective: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an enzyme with several pro-inflammatory properties that involved in pathogenesis of atherosclerosis, but some investigation shows controversial views regard...
POTENTIAL NATURAL PRODUCTS WITH ANTICANCER PROPERTIES AND THEIR APPLICATIONS
Natural products continue to be a source for the discovery of drugs and drug leads even from ancient period. 80% of drug molecules have been obtained from either natural products or derivatives of the natural product. It...
ANTIOXIDANT POTENTIAL PROFILE OF PAJANELIA LONGIFOLIA (WILLD.) K. SCHUMAN.; POTENTIAL NEW SOURCES OF NATURAL ANTIOXIDANT.
Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extracts of Pajanelia longifolia (Willd.) K. Schuman, ethnomedicinally prescribed plant as hepatoprotective. Methods: Bark ext...
DRUG UTILIZATION PATTERN AMONG GERIATRIC PATIENTS ADMITTED IN MEDICAL INTENSIVE CARE UNIT IN A TERTIARY CARE TEACHING HOSPITAL
Objective: This study aims to evaluate the drug utilization pattern in terms of defined daily dose (DDD) among geriatric patients admitted in medicalintensive care unit (MICU) of a tertiary care hospital.Methods: Retrosp...
A NEW REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE SIMULTANEOUS ESTIMATION OF SERRATIOPEPTIDASE AND DICLOFENAC SODIUM IN BULK AND TABLET DOSAGE FORM
Objective: The objective is to study the development of a simple, rapid, specific, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of serratiop...