Assessment of Cytotoxic Activity of Calotropis Procera’s Extracts Using Artemia Salina Assay
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 18, Issue 5
Abstract
The essence of the present study is to focus on the cytotoxicity of the aqueous and solvent (acetone, methanol and ethanol) of the medicinal plant (Calotropis procera) belonging to the family Apocynaceae. Brine shrimp lethality bioassay method was established for the present study and the cytotoxicity was reported in terms of lethality concentration (LC50). The shrimps were hatched and active shrimps were collected and used for the assay. Ten active naupilli were added to the 1ml of the diluted extracts, concentration was varied from 10, 100, 1000µg/ml. The surviving (larvae) shrimps were counted after 24hrs, 48hrs and lethality concentration LC50 was also assessed. It was observed that the methanol extract of Calotropis procera leaf have weak cytotoxic compounds while other extracts were able to cause 45% mortality of brine shrimp larvae after 48hours. The present study supports that brine shrimp bioassay is simple, reliable and convenient method for assessment of bioactivity and cytotoxicity of medicinal plants. The cytotoxicity exhibited by the crude extracts were promising and this indicates the presence of potent bioactive compounds.
Authors and Affiliations
Asoso O S, Oladunmoye M K, Ogundare AO
Keywords
Related Articles
- EP ID EP622319
- DOI 10.26717/BJSTR.2019.18.003224
- Views 126
- Downloads 0
Review on Application of Agro-Waste Biomass Biochar for Adsorption and Bioremediation Dye
This review brings the little knowledge about the effectiveness of combined bio-film and biochar approach in bio-sorption method for the removal of synthetic dye which generated from wastewater of textile and carpet indu...
Bringing Food and Nutrition Information to Filipinos in Just a Click of the Finger Through iFNRI: The Potentials of Information Technology to Inform, Educate and Serve
With the current internet era, people have been searching online for health and nutrition information. Hence, the public need access to accurate and appropriate diet and nutrition information, which could help improve on...
Goldenhar syndrome: An oculo-auriculo-mandible syndrome was reported
In A 2-month-old girl with suffer from eye-facial problems was referred to our clinic. The baby was full-term [1,2] delivery without complications. She had limbal dermoid of left eye with 3mm diameter and both eyes with...
Bacteria from Marine Sponges: A Source of Biologically Active Compounds
The Red Sea marine sponges have been investigated for isolating novel bacterial strains. The main objective of the present study is to carry out a molecular level identification of the isolated sponge-associated bacteria...
Yellow Wasp Polistes Flavus Venom Protein, Its Purification, Solubilization and Antimicrobial Activity
Yellow wasp Polistes flavus venom toxins were isolated by cutting the last two segments of abdominal region and purified on Sepharose CL- 6B 200 column, the molecular weight of venom protein from yellow wasp ranging from...