Yellow Wasp Polistes Flavus Venom Protein, Its Purification, Solubilization and Antimicrobial Activity
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2017, Vol 1, Issue 1
Abstract
Yellow wasp Polistes flavus venom toxins were isolated by cutting the last two segments of abdominal region and purified on Sepharose CL- 6B 200 column, the molecular weight of venom protein from yellow wasp ranging from 6-70kD. The purified venom toxins were investigated for its antimicrobial activities against three infectious bacterial pathogens. Paper disc diffusion and serial dilution assays were performed for determination of Inhibition Zone Diameter (IZD) and Minimal Inhibitory Concentration (MIC) respectively. MIC values obtained were 12.3 μg/ ml against E. coli, and 24.69 μg/ml for Salmonella typhi and Vibrio cholera. By agar disc diffusion method, the Inhibition Zone Diameter (IZD) for E. coli, Salmonella typhi and Vibrio cholera were 36±0.14, 19.96±0.36 and 21.69±0.22 respectively. In present investigation, Triton X-100 was found good solubilizing agent for venom protein. The antibacterial effects of wasp venom may be due to the action on cell membrane destruction and cell-lyses. Hymenopteran insects mainly wasps, honey bee, hornets inflicts venom to maintain self-defense for protection of territory. Hymenopteran venoms are secreted from poison glands which are attached to a sting apparatus present in last abdominal segment. It serves both as defensive substances against aggressors as well as weapon used to paralyze the victim during gaining food. Wasps react and respond very fast to make an attack on predators and mammals. It is also highly toxic to micro-organisms. Venom toxins have been evolved to capture prey and make defense against predators and/or micro-organisms. Generally, wasp envenomation occurs after little disturbance near the hive, they inflict venom into the body of mammals mainly man and his pets [1]. Wasps inflict venom for making self defense. Chemically the venom protein of wasp is the mixture of biologically active substances of highmedium and small molecular weight with a variety of physiological function [2]. Wasp venom is a complex mixture of several hundreds of different components and lethal toxins. It is the complex mixture of active amines such as serotonin, histamine, tyramine, dopamine, etc and peptides such as kinins, mastoparans, crobroline etc. and many types of enzymes such as hydrolases (proteases, hyaluronidase, phospholipases, phospholipases A, nucleiotidases) and as well as allergens [3] and toxins [4]. Wasp venom is highly toxic to small mammals in which it causes tissue irritation, swelling, redness, inflammation and pain. The wasp toxin induce severe anaphylaxis in animals and man soon after stinging [5] and cause early and delayed hypersensitivity, inflammatory reaction, necrosis and toxic complications in mammals and even invertebrates animals [6]. Venom toxins of Polybia poulista showed genotoxic and mutagenic effects. Polyamines toxins from yellow wasp is potent openchannels blockers of ionotropic glutamate receptors that shows selective ligand binding [7].
Authors and Affiliations
Krishna Kumar Prajapati, Ravi Kant Upadhayay
Keywords
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- EP ID EP567436
- DOI 10.26717/BJSTR.2017.01.000141
- Views 173
- Downloads 0
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