Assessment of physical damage of cryopreserved RBCs during thawing by impedance spectroscopy
Journal Title: International Journal of Medical Science and Public Health - Year 2015, Vol 4, Issue 8
Abstract
Background: Preservation of red blood cells (RBCs) is an important task to ensure a long-term, readily available, safe blood supply for transfusion during emergency. Effective preservation procedures are required at various steps for the long-term storage of an RBC product including testing, inventory, quality control, and product distribution. Biopreservation is the process of maintaining the integrity and functionality of cells outside the native environment for long storage times. Hypothermic storage, cryopreservation, and lyophilization are the various methods of biopreservation. Objective: To monitor the physical integrity of erythrocytes after cryopreservation with the help of impedance spectroscopy. Materials and Methods: Cryoprotectants were added to protect the erythrocytes from cryoinjuries. In addition, the eryth - rocytes viability was monitored through hemolysis test. Afterward, the exact numbers of live or lysed cells were observed by the effect of thawing rate on the cells through impedance spectroscopy, as we know that there is a relationship between impedance, frequency, and temperature. Result: As the frequency increases, the impedance decreases, which indicates that the RBCs are lysed. The impedance starts decreasing at −200 °C and drastically decreased at −140 °C. Conclusion: This study indicates the real viability of the RBCs through impedance measurement by the impedance spectroscopy
Authors and Affiliations
Jyoti Srivastava, Shankar Khade
Keywords
Related Articles
- EP ID EP90047
- DOI 10.5455/ijmsph.2015.16032015253
- Views 149
- Downloads 0
Acute effect of uphill and downhill treadmill walk on cardiovascular response and perceived exertion in young sedentary individual
Background: Systematic exercise is an essential component for health promotion and improvement of the quality of life. Exercise that emphasizes on the eccentric (lengthening) skeletal muscle contractions has recently gai...
Association between serum ferritin and thyroid hormone profile in hypothyroidism
Background: Ferritin is an iron storage protein found in almost all of the body tissues. Serum ferritin levels also have been reported to be altered in patients with thyroid disease. Thus, changes in the serum concen...
The etiological spectrum of pancytopenia diagnosed from blood smears examined in the pathology department of a rural tertiary care centre in South India
Background: Pancytopenia is a reduction in all three formed elements of blood, namely - erythrocytes, leukocytes, and platelets. This triad of findings occurs in a number of clinical conditions. The underlying pathology...
A population-based cross-sectional study on consanguineous marriages in rural Tamil Nadu, India
Background: Consanguineous marriages were common particularly in the south India. Though there were studies on consanguineous marriages, only a few were population-based studies. In this context, it was important...
Cancer pattern among newly registered patients in a cancer hospital of South Gujarat
Background: Cancer is a chronic disease, its prevalence is increasing worldwide due to various advances exist for the diagnosis and treatment. Cancer occurs as a result of unusual growth of cells and invasion of nearby t...