Autologous Serum as Growth Factor Substitute for Isolation and Characterization of Lung Cancer Stem Cells: an In-vitro Approach
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2016, Vol 18, Issue 1
Abstract
Cancer stem cells (CSCS), comprising of a very small subpopulation of cells within the tumor mass, has been increasingly reported to be responsible for initiation, progression, relapse and metastasis. Attributed to these characteristics, lung cancer is now known to be a stem cell disease. Thus, the need of the hour is to generate inter- and intra-tumoral (LCSC) models on the basis of their cancer initiating property from a heterogeneous population and isolate lung cancer stem cells (LCSCs) by using multiple marker approach. Therefore, in the present study, heterogeneous population of LCSCs were isolated from a single cell suspension of non small cell Lung cancer (NSCLC) tissue and enriched in serum-free culture as well as in media containing patient’s own serum to form pleurospheres (PS). In both conditions, PS exhibited an enhanced capacity for self-renewal and resistance towards chemotherapeutic drug; however, a significant increase was observed in the size of PS enriched in the patient’s own sera after the second passage. Furthermore, results of our study demonstrated that apart from CD133, an established CSC maker in NSCLC, ALDH and EpCAM may also serve as a putative cell surface marker for lung adenocarcinoma stem cells. Thus, culturing NSCLC cells in the presence of autologous serum could serve as an effective in-vitro model for enriching LCSCs by mimicking the in-vivo microenvironment conditions and facilitating the growth for future gene and stem cell targeted therapies. Additionally, isolation and characterisation of LSCSs by using multiple marker approach proves to a better method than the conventional single marker based approach which tends to underestimate presence of other putative LCSC in NSCLC. Collectively, this approach can be used to translate the basic research findings like invasive properties, drug screening, chemoresistance, apoptosis, immune responses, proliferation, gene expression and targeting various signalling pathways into clinical trials.
Authors and Affiliations
Sheefa Mirza, Kanisha Shah, Harsha Panchal, Nayan Jain, Rakesh Rawal
Keywords
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