Bactericidal effect of nd: yag laser irradiation on enterococcus faecalis – ex vivo study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 2
Abstract
The aim of this study is to define the role of NdYAG laser in root canal disinfection with minimally invasive concept. The hypothesis was tested ex vivo that NdYAG laser irradiation has a bactericidal effect on E. faecalis inoculated in instrumented root canals. Methodology includes observation of bacterial cell structural changes using CSEM and ESEM on inoculated dentin surfaces following direct and indirect NdYAG laser irradiation respectively along with resultant colony forming unit count. Results showed NdYAG laser irradiation of E. faecalis inoculated canals resulted in significant reduction (p<0.005 wilcoxon signed rank test) of E. faecalis which meant 97.3% eradication of microbes (both conventional SEM and environmental SEM) also proves the NdYAG laser has a bactericidal effect on E. faecalis(cell structural changes in E.faecalis and disruption of biofilm) upto 1mm of thickness of dentin and dentinal tubules. To conclude Nd:YAG laser irradiation is an adjunct and not an alternative to various disinfectant protocols particularly in repeated failure of nonsurgical endodontic treatment and in persistent infections of pathogen like E. faecalis which resists many antimicrobial treatment, however E. faecalis that grow as biofilm are quite difficult to eradicate totally even on direct laser treatment.
Authors and Affiliations
Amudha lakshmi, Dr. A. Nandini MDS, Dr Dhanalakshmi Subramanian, Dr. P. Shakuntala
Keywords
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- EP ID EP467733
- DOI 10.9790/0853-1802141826.
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