Benz[a]anthracene Decreases Plasma Calcium Levels Resulting from Influence of Scale Osteoclastic and Osteoblastic Activities in Goldfish
Journal Title: International Journal of Zoological Investigations - Year 2017, Vol 3, Issue 1
Abstract
In the present study, the effects of polycyclic aromatic hydrocarbons (PAHs) on plasma calcium, osteoblasts and osteoclasts were investigated. Goldfish were intraperitoneally injected with benz[a]anthracene (BaA) (5μg/g body weight) (around 10-5 M). BaA induced hypocalcemia at 24 and 48 h and thereafter the level recovered to control levels at 72 h. Goldfish scales possess both osteoclasts (bone resorption cells) and osteoblasts (bone forming cells). The marker enzyme activity (tartrate-resistant acid phosphatase: TRAP) of osteoclasts in goldfish scales decreased at 12 and 24 h after BaA injection. In BaA injected goldfish scales, the marker enzyme (alkaline phosphatase, ALP) gradually decreased at 48 and 72 h. In addition, 4-hydroxybenz[a]anthracene (4-OHBaA) that is one of the metabolites of BaA by conversion enzyme (P4501A1) was detected in the bile of goldfish at 12, 24, 48, and 72 h after administration of BaA to goldfish. We have recently found the toxicity of monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs), metabolites of PAHs, in bone metabolism. We reported that 4- OHBaA suppressed both TRAP and ALP activities in the cultured scales of goldfish. Therefore, it was prob able that 4-OHBaA metabolized from BaA has toxicity for osteoclasts and osteoblasts in goldfish. These phenomena are a cause of the disruption of the bone metabolism and the induction of spinal deformities.
Authors and Affiliations
Nobuo Suzuki
Keywords
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