[b]Optimization of Culture Parameters for Maximum Polyhydroxybutyrate Production by Selected Bacterial Strains Isolated from Rhizospheric Soils[/b]
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 3
Abstract
The enormous applications of conventional non-biodegradable plastics have led towards their increased usage and accumulation in the environment. This has become one of the major causes of global environmental concern in the present century. Polyhydroxybutyrate (PHB), a biodegradable plastic is known to have properties similar to conventional plastics, thus exhibiting a potential for replacing conventional non-degradable plastics. In the present study, a total of 303 different bacterial isolates were obtained from soil samples collected from the rhizospheric area of three crops, viz., wheat, mustard and sugarcane. All the isolates were screened for PHB (Poly-3-hydroxy butyric acid) production using Sudan Black staining method, and 194 isolates were found to be PHB positive. Based upon the amount of PHB produced, the isolates were divided into three categories: high, medium and low producers. Representative isolates from each category were selected for biochemical characterization; and for optimization of various culture parameters (carbon source, nitrogen source, C/N Ratio, different pH, temperature and incubation time periods) for maximizing PHB accumulation. The highest PHB yield was obtained when the culture medium was supplemented with glucose as the carbon source, ammonium sulphate at a concentration of 1.0 g/l as the nitrogen source, and by maintaining the C/N ratio of the medium as 20:1. The physical growth parameters which supported maximum PHB accumulation included a pH of 7.0, and an incubation temperature of 30 °C for a period of 48 h. A few isolates exhibited high PHB accumulation under optimized conditions, thus, showing a potential for their industrial exploitation.
Authors and Affiliations
Priyanka Lathwal, Kiran Nehra, Manpreet Singh, Pragati Jamdagani, Jogender S. Rana
Keywords
Related Articles
- EP ID EP81155
- DOI -
- Views 83
- Downloads 0
Early KPC-Producing Klebsiella pneumoniae Bacteremia among Intensive Care Unit Patients Non-Colonized upon Admission
Among 140 patients colonized by KPC-producing Klebsiella pneumoniae (KPC-Kp) between fourth and seventh day of Intensive Care Unit stay, 24 developed bacteraemia immediately after colonization. Colistin-resistance of the...
Relationship between ureB Sequence Diversity, Urease Activity and Genotypic Variations of Different Helicobacter pylori Strains in Patients with Gastric Disorders
Association of the severity of[i] Helicobacter pylori [/i]induced diseases with virulence entity of the colonized strains was proven in some studies. Urease has been demonstrated as a potent virulence factor for [i]H. py...
Seroprevalence of Rubella and Cytomegalia in Young Women from Biała Podlaska District
The aim of this study was to analyze the seroprevalence of rubella and cytomegalia among young women. The study included 175 healthy women from the Biała Podlaska District, aged 16 to 35 years. Anti-rubella and anti-CMV...
Streptococcus anginosus (milleri) group strains isolated in Poland 1996-2012 and their antibiotic resistance patterns
[i]Streptococcus anginosus[/i], [i]Streptococcus intermedius[/i] and [i]Streptococcus constellatus[/i] form group of related streptococcal species namely Streptococcus Anginosus Group (SAG). The group, previously called...
Chaperone DnaJ Influences the Formation of Biofilm by Escherichia coli
DnaJ chaperone, a member of the so called DnaK-DnaJ-GrpE chaperone machine plays an important role in cell physiology. The ability of Escherichia coli ΔdnaJ mutant to form biofilm was studied. It was shown that this muta...