Breeding of High Natamycin-producing Strains and Optimizing of Fermentation Process

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Breeding of High Natamycin-producing Strains and Optimizing of Fermentation Process

Journal Title: 河南科技大学学报（自然科学版） - Year 2016, Vol 37, Issue 4

Abstract

Streptomyces natalensis 4.3505 was used as the original bacterial strain and the ultravoilet ray was employed as the mutation method. In order to filter out high-yield Natamycin mutant strain for anti-sodium acetate,the fermentation conditions were optimized. The optimal elicitors and the adding techniques were investigated. The results show that the mutant strain is S. natalensis HW-2 with good genetic stability,and the yield of natamycin reaches to 452. 2 mg / L for 120 h fermention,which is increased 242. 6% compared with the original strain. The optimal fermentation conditions of natamycin obtained by S. natalensis HW-2 are 6% inoculum concentration,30 m L volume content in 250 m L triangular bottle and initial pH of 7. 5. The best precursor is sodium acetate,and the mass fraction and adding time is 0. 2% and 24 h after fermentation,respectively.

Authors and Affiliations

Shuaiying ZHANG, Lanlan WEI, Ying ZHANG, Jia HE, Dahong WANG

Keywords

Streptomyces natalensis; Natamycin; mutation breeding; fermentation conditions;

EP ID EP477163
DOI 10.15926/j.cnki.issn1672-6871.2016.04.017
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