Callus formation of sugar cane var. ‘SP 70-1284’ in liquid culture medium from field explants
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 1
Abstract
Explants from immature sections of young leaves in the stems of the variety of sugarcane ‘SP 70-1284’, were taken directly of field whit the objets of study the capacity for organogenic callus formation. The effect of the concentration of 2,4-D in the callus formation was evaluated using liquid medium and considering the section of the explante utilized. The best callus formation and quality of them was achieved in the three first sections nearly to the apical meristem whit the use of 3 mg.l -1 of 2, 4-D. With these explantes the lowes indices of contamination and fenolization were obtained.
Authors and Affiliations
Apolonio Valdez Balero , Pedro A. Orellana Pérez , Novisel Veitía Rodríguez, Damaris Torres Rodríguez
Keywords
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