cDNA cloning of porcine CDH1 and its expression profile in porcine early parthenotes
Journal Title: Animal Science Papers and Reports - Year 2012, Vol 30, Issue 3
Abstract
The study aimed at cloning the complete cDNA sequence of porcine CDH1 gene and detecting its expression in different developmental stages of early parthenotes. After cloning the CDH1 gene from porcine oviduct using rapid amplification of cDNA ends (RACE) method, the sequence analysis revealed that porcine CDH1 gene complete cDNA nucleotide sequence amounted to 4283 bp including 2652 bp of open reading frame (ORF), 105 bp of 5’ untranslated region (UTR) and 1526 bp of 3’ UTR. The ORF encoded a deduced protein precursor of 97 kD with 883 amino acid residues. The precursor protein including signal peptide, extracellular region, membrane-spanning region and cytoplasmic region had a single transmembrane structure, and its extracellular region had HAV motif and some Ca2+ binding regions. The porcine CDH1 protein showed high homology with cattle (89%), horse (87%), dog (86%), human (84%), chimpanzee (83%) and mouse (83%) CDH1. The results from RT-PCR and real-time PCR indicated that CDH1 gene could be expressed in both immature and mature oocytes, as well as early parthenotes (2-, 4-, 8-cell embryos, morula, blastocysts). The data of real-time PCR showed that the expression of CDH1 was the highest at 2-cell parthenogenetic embryos stage, and then it decreased significantly till 8-cell embryos stage; the second increased expression level occurred at morulas stage, and then also showed a diminishing trend in the following developmental stage. This is the first report of cloning and analysing the porcine CDH1 cDNA that provides critical information for further research of its functions in pig embryo development.
Authors and Affiliations
X. G. Yang, X. W. Wang, E. W. Zuo, C. L. Hao, S. L. Lu, H. B. Liu, H. Y. Hu, Y. Q. Lu, K. H. Lu
Keywords
Related Articles
- EP ID EP70894
- DOI -
- Views 139
- Downloads 0
Associations between polymorphism in the 2,4-Dienoyl-CoA Reductase 1 gene (DECR1) and growth traits of Shanxi White pig
A polymorphism within exon 2 of the 2, 4-dienoyl-CoA reductase gene (DECR1) was investigated by PCR-SSCP in 228 Shanxi White pigs. An association between the DECR1 polymorphism and growth traits in Shanxi White pigs was...
Lipid profile of intramuscular fat in lamb meat
Lipid profile of intramuscular fat (IMF) from semitendinosus muscle of lambs was examined in relation to the intensity of fattening system, animals’ final body weight and sex. Conducted were two repeations of fattening (...
Effect of morphine on activity of five selected lysosomal enzymes in liver and kidneys of mice
The experiment was carried out on 90 Swiss male mice divided into 9 groups (n=10). Over 4, 10 and 14 days mice of three control groups (I-III) were injected with 250 μl 0.9% NaCl solution daily, and those from six experi...
Adaptation of primer extension method for monitoring of scrapie-associated genotypes in sheep PRNP gene
The paper describes adaptation of primer extension method for genotyping scrapie-associated codons 136, 141 and 156 in sheep PRNP gene. As the method failed to genotype the polymorphism in codon 171 in some samples, this...
How “natural” training methods can affect equine mental state? A critical approach – a review
Among equestrians the “natural” training methods of horses are gaining widespread popularity due to their spectacular efficiency. Underlying philosophy of trainers - founders of different “natural horsemanship training”...