cDNA-SRAP ANALYSIS OF DIFFERENTIAL GENE EXPRESSION IN FOXTAIL MILLET (Setaria italica L.) UNDER DROUGHT STRESS
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 1
Abstract
Foxtail millet (Setaria italica L.) is an important food and fodder grain crop in arid and semi-arid regions of Asia and Africa. Being an elite drought-tolerant crop, foxtail millet is thought to be an excellent experimental model in studying abiotic stress tolerance system. The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression in Setaria italica L. cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression in foxtail millet i.e. Setaria italica L. under drought stress. Analysis on the expression profiling of genes revealed that the expression of genes was upregulated by water stress treatment, suggesting that they may involve in the drought tolerance of the crop. This study constitutes the first report that opens up the application of cDNA-SRAP in finding new gene involved in drought tolerance in Setaria italica. Further efforts in validation of identified genes could be explored in development of tolerant lines by MAS as a functional marker and can also be used as candidate genes for development of water stress tolerant transgenics in other related crops.
Authors and Affiliations
DIPTI GAWAI, M. P. MOHARIL, P. V. JADHAV, S. J. GAHUKAR
Keywords
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