Changes in Function and Expression Level of Multidrug Resistance-Associated Protein during Intestinal Ischemia/Reperfusion
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 7, Issue 2
Abstract
Abstract: Ischemic bowel disease is induced by poor circulation of blood, and thus oxygen and nutrition necessary for the small intestine are not supplied enough. As a result, such condition causes inflammation and ulcers, such as ischemic colitis and acute mesenteric arterial occlusion. We previously reported changes in function and expression level of P-glycoprotein (P-gp) which is a typical and important ABC transporter in the small intestine, liver and kidney during intestinal ischemia/reperfusion (I/R). In this study, we examined I/R-induced changes in multidrug resistance-associated proteins (Mrps) which is also an important ABC transporter like P-gp. The superior mesenteric vein and artery in rat were occluded by hanging itself using surgical-sutures connected with the spring balance for 60 min, followed by a reperfusion. The excretion clearances to jejunal lumen (CLjejunum), bile (CLbile) and urine (CLurine) of 5(6)-carboxyfluorescein (5-CF) diacetate (5-CFDA), a Mrp substrate, were determined after i.v. administration of 5-CFDA. We also investigated changes in the protein and mRNA expression levels of Mrp2 during intestinal I/R. It was shown that the expression behavior of Mrp2 is different depending on each organ during intestinal I/R. For the function of Mrp, during early term of reperfusion, the total clearance (CLtot) of 5-CFDA (5-CF) was significantly decreased by intestinal ischemia compared with the control condition. However, the behavior of excretion clearance was differ depending on each organ. In longer term of reperfusion, CLtot of 5-CFDA (5-CF) of intestinal I/R condition was converged to the control level until 24 hr after reperfusion. Intestinal I/R change the function and expression level of intestinal Mrp2 as well as hepatic and renal Mrp2. Therefore, when performing drug therapy to the ischemic bowel disease, the caution is necessary for the dosing of Mrp2 substrates.
Authors and Affiliations
Yusuke Takizawa, Nasa Sakamoto, Masahiro Hayashi
Keywords
Related Articles
- EP ID EP479689
- DOI -
- Views 107
- Downloads 0
Role of Uric Acid in Detection of Early Renal Function Decline in Type 1 Diabetes
Abstract:Recent studies have reported that a high serum level of uric acid is strongly associated with impaired renal function and cardio vascular disease. This study aims to find out the role of uric acid in detection o...
Spirulina-The Nature’s Wonder: A Review
Abstract: Spirulina is a fresh water microalgae which has been used as a food supplement since many years. This alga contains a diverse concentration of nutrients and has emerged as a wonder drug because of its varied us...
Comparison of Clonidine and Dexmedetomidine for Attenuation of Hemodynamic Responses of Intubation and Pneumoperitoneum During Laparoscopic Cholecystectomy: A Randomized Double Blind Placebo Controlled Trial
Hemodynamic derangements, associated with intubation and pneumoperitoneum during laparoscopic surgery can be attenuated by use of α2 agonist drugs like clonidine and dexmedetomidine. To compare the attenuating effects of...
To Study the Effects of Various Fixatives on Liver – A Histological Study
Abstract: Thefixation also in optical differentiation of cells and tissue constituents by altering their refractive indices in varying degrees. This is of value in the microscopic examination of cells and tissue.The pres...
An Unusual Case of Primary Actinomycosis of the Hand: Case Report and Literature Review
Actinomycosis is a rare endogenous infection and its manifestation in the extremities is even less reported in literature. In fact, it mostly involves cervical-facial area, and then come thoracic and abdominal locations....