Chaperon DnaJ Influences the Formati[i][/i]on of Biofilm in [i]Escherichia coli[/i]
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 3
Abstract
DnaJ chaperone, a member of the so called DnaK-DnaJ-GrpE chaperone machine plays an important role in cell physiology. The ability of [i]Escherichia coli ΔdnaJ[/i] mutant to form biofilm was studied. It was shown that this mutant is impaired in biofilm development when exposed to 42°C for 2 h. The impairment in biofilm development was observed when the heat shock was applied either at the onset of biofilm formation or 2 h later. The biofilm formed was thinner and its structure was changed as compared to wild-type strain. This defect could be complemented by the introduction of a wild-type gene on a low-copy plasmid.
Authors and Affiliations
Anna Grudniak, Jolanta Włodkowska, Krystyna Wolska
Keywords
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