Participation of TP53 and Bcl-2 proteins in gastric carcinomas associated with [i]Helicobacter pylori[/i] or Epstein-Barr virus (EBV)
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 3
Abstract
In the presented studies p53 and bcl-2 proteins expression were evaluated in samples of gastric carcinomas in patients with [i]Helicobacter pylori[/i] or EBV or without [i]H. pylori[/i]/EBV infection. The studies were conducted on 64 adult patients with gastric adenocarcinomas: 16 patients with [i]H. pylori [/i]([i]cagA[/i]+)-positivity (group 1), 14 with EBV-positive tumours (group 2), 12 with [i]H. pylori[/i]/EBV-positive tumours (group 3) and 22 patients with [i]H. pylori[/i]/EBV-negative tumours (group 4). [i]H. pylori [/i]presence in gastric tumour specimens was detected using Giemsa staining and bacterial culture technique. Moreover, [i]cagA[/i] gene was detected using PCR. EBV infection was detected based on EBER presence in the tissue by RNA in situ hybridization. Expressions of p53 and bcl-2 proteins were analysed using immunohistochemistry. Expression of p53 was noted in 14 (84%) patients from group 1, 8 (57%) patients from group 2, 7 (58%) patients from group 3, and 19 (86%) patients from group 4, whereas expression of bcl-2 was noted in 12 (75%) patients from group 1, in 10 (71%) patients from group 2, 9 (75%) patients from group 3, and 6 (27%) patients from group 4. The obtained results allow the conclusion, that [i]H. pylori [/i]([i]cagA[/i]+)-associated development of the gastric adenocarcinoma is determined by abnormalities in the p53 protein function and overexpression of anti-apoptotic bcl-2 protein, whereas EBV-associated adenocarcinomas seem to be related with apoptosis resistance associated with bcl-2 overexpression.
Authors and Affiliations
Andrzej Szkaradkiewicz, Tomasz Karpiński, Jan Majewski, Kamila Malinowska, Olga Goślińska-Kuźniarek, Krzysztof Linke
Keywords
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