Characterization and Antimicrobial Activity of AgNPs Synthesized by Streptomyces sp. RHS16 Against Fish Pathogens
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 4
Abstract
Silver nanoparticles (AgNPs) attracted great attention due to their unusual and fascinating properties, and applications in many fields. This work reports the fabrication of AgNPs by a newly isolated Streptomyces sp. RHS16. The synthesis of silver nanoparticles was mediated by nitrate reductase enzyme secreted in the culture filtrate of the bacterium and detected using UV-visible spectra that showed a strong and broad peak between 420 nm and 450 nm. The effect of crude enzyme concentration and silver nitrate molarity was evaluated. As the volume of crude enzyme increased from 1ml to 4ml, the color changed to dark reddish brown and the absorbance values increased while the color intensity increased with the increase in molarity of silver nitrate. FTIR spectral analysis of St.sp.R16 AgNPs showed an array of absorbance bands at 3422.36, 2922.88, 2393.31, 1756.94, 1631.62, 1384.06, 1035.10, 823.03, 754.58, 695.08 and 540.54 cm−1 . EDX spectrum revealed a strong signal in the silver region and confirmed the formation of metallic silver nanocrystals with typical optical absorption peaks approximately at 3 KeV. The characteristic XRD peaks were centered at ∼ 38.02◦, ∼ 44.44◦ and ∼ 64.51◦, 77.68◦, and 81.35◦ which could be induced by the following crystalline planes of silver; (111), (200), (220), (311), and (222), respectively. TEM showed that size of the silver particles were in the range of 1–15 nm. The Ag nano flowers (AgNFs) of average size in between 1 to 133 nm were observed with SEM. The nanoparticles possessed antimicrobial activity against some fish bacterial pathogens with strong activity against Candida albicans (inhibition zone of 18 mm). Keywords: Silver nanoparticles, Streptomyces sp. RHS16, Biosynthesis, Antimicrobial activity, Fish pathogens, XRD, TEM, SEM, EDX, and FTIRSilver nanoparticles (AgNPs) attracted great attention due to their unusual and fascinating properties, and applications in many fields. This work reports the fabrication of AgNPs by a newly isolated Streptomyces sp. RHS16. The synthesis of silver nanoparticles was mediated by nitrate reductase enzyme secreted in the culture filtrate of the bacterium and detected using UV-visible spectra that showed a strong and broad peak between 420 nm and 450 nm. The effect of crude enzyme concentration and silver nitrate molarity was evaluated. As the volume of crude enzyme increased from 1ml to 4ml, the color changed to dark reddish brown and the absorbance values increased while the color intensity increased with the increase in molarity of silver nitrate. FTIR spectral analysis of St.sp.R16 AgNPs showed an array of absorbance bands at 3422.36, 2922.88, 2393.31, 1756.94, 1631.62, 1384.06, 1035.10, 823.03, 754.58, 695.08 and 540.54 cm−1 . EDX spectrum revealed a strong signal in the silver region and confirmed the formation of metallic silver nanocrystals with typical optical absorption peaks approximately at 3 KeV. The characteristic XRD peaks were centered at ∼ 38.02◦, ∼ 44.44◦ and ∼ 64.51◦, 77.68◦, and 81.35◦ which could be induced by the following crystalline planes of silver; (111), (200), (220), (311), and (222), respectively. TEM showed that size of the silver particles were in the range of 1–15 nm. The Ag nano flowers (AgNFs) of average size in between 1 to 133 nm were observed with SEM. The nanoparticles possessed antimicrobial activity against some fish bacterial pathogens with strong activity against Candida albicans (inhibition zone of 18 mm).
Authors and Affiliations
Rana F. El Baradaei, Samia S. Abouelkheir, Hanan A. Ghozlan, Soraya A. Sabry
Keywords
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