CHEMICAL AND BIOLOGICAL STUDY OF EUGENIA STIPITATA MC VAUGH COLLECTED IN THE COLOMBIAN ANDEAN REGION
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objective: The main objective of this study is to evaluate the chemical and bioactive properties of Eugenia stipitata (Myrtaceae) fruit seeds collected in the Andean region of Colombia using an ethanolic extract, a dichloromethane fraction (DF), and a hydroalcoholic residue.Methods: E. stipitata seeds were evaluated using bromatological analysis; these were macerated (ethanol 96%) and partitioned (dichloromethane). Phytophenols composition of the samples was tested by high-pressure liquid chromatography-mass spectrometry and Folin–Ciocalteu method, then calculated as gallic acid equivalents (GAEs). Antiradical capacity was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 3-ethylbenzthiazoline-6- sulfonic acid (ABTS•+) and inhibitory concentration 50% (IC50) was calculated; antioxidant capacity was determined using ferric reducing antioxidant power, oxygen radical absorbance capacity, and OH• radical and hemolysis inhibition assays. Anthelmintic activity against Panagrellus redivivus and ovine gastrointestinal (GI) nematodes and toxicity on human blood cells were tested.Results: E. stipitata seeds contain protein, sulfur, boron, iron, and copper. The ethanolic extract exhibited significant anthelmintic activity against free-living and GI nematodes, which would be associated with the phytophenols content, mostly tannins (29.14 g GAE/100 g). DF displayed the highest antiradical capacity in DPPH• and ABTS•+ (IC50: 2.65 and 0.33 mg/l, respectively) methods, antioxidant capacity, and hemolysis inhibition of human erythrocytes (IC50: 200 mg/l). There were no toxic metabolites to human blood cells.Conclusion: These findings would be useful for promoting the use of E. stipitata seeds, collected in the Colombian Andean region, in biotechnological processes. This is the earliest paper showing a study carried out in Colombia with an interest in evaluating chemical and biological characteristics of E. stipitata seeds.
Authors and Affiliations
Andree Ãlvarez, Ãngel JimÉnez, Jonh Méndez, Elizabeth Murillo
Keywords
Related Articles
- EP ID EP605226
- DOI 10.22159/ajpcr.2018.v11i12.27253
- Views 102
- Downloads 0
ANTIDIABETIC ACTIVITY OF KALANCHOE PINNATA IN ALLOXAN-INDUCED DIABETIC RATS
Objective: The main purpose of the present study was to analyze the antidiabetic activity of methanolic extract of the leaf of Kalanchoe pinnata in alloxan-induced diabetic rats. Methods: Diabetes mellitus (DM) is a chr...
PREDICTORS OF MAJOR BLEEDING AMONG ATRIAL FIBRILLATION PATIENTS ON WARFARIN
Objective: Bleeding is the most serious complication associated with anticoagulation therapy. The purpose of this study was to estimate the frequency of major bleeding related to warfarin and to identify its predictors i...
REVIEW OF MEDICINAL USES, PHYTOCHEMISTRY, AND PHARMACOLOGICAL PROPERTIES OF DRIMIA ELATA
Drimia elata is an important and well-known medicinal plant in tropical Africa. This study critically reviewed the medicinal applications, phytochemistry, and pharmacological activities of D. elata. Literature on medicin...
IN VITRO EVALUATION OF ANTIOXIDANT-MEDIATED APOPTOSIS ON CANCER CELLS AND NON-TOXICITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ AND PYROGALLOL
Objective: Antioxidants are very important for human health, and hence, external supplementation is recommended to protect cells from the deleterious effects of excessive reactive oxygen species conditions that are capab...
CHRONIC TENSION-TYPE HEADACHE: MUSCLE OVERACTIVITY VERSUS DEFICIENT PAIN CONTROLS
Objective: The oscillating school of thought between the peripheral and central mechanisms of pain origin hinders the selection of appropriate therapy for chronic tension-type headache (CTTH). The aim of this study was t...