Chemiluminiscnce and tumor markers
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2016, Vol 2, Issue 6
Abstract
This observation revolves around the chemiluminescence biomarkers These are applied for validation methods and screening. Here, was revised the development of the new analytical tool through immunological (antibody) and chemiluminescent methods combination, named chemiluminescence immunoassay (CLIA). In the tumoral process,increased expression of specific antigens is associated in patients with certain tumors. Thus, in our laboratories CLIA has been tested to study different tumoral lesions from glandular tissues, like prostate and thyroid. A sensitive chemiluminescence (CL) imaging immunoassay method for detection of multiple tumor markers with high throughput, easy operation, and low cost was developed. The immunosensor array was prepared by covalently immobilizing capture antibodies on corresponding sensing sites on a silanized disposable glass chip. Gold nanoparticle-based bioconjugates with a high molar ratio of horseradish peroxidase (HRP) to detection antibodies were used for signal amplification. Under a sandwich immunoassay, the CL signals triggered by HRP captured on each sensing cell were collected by a charge-coupled device for simultaneous measurement of biomarkers and combination diagnosis of certain tumors. As a proof of concept, the immunosensor array was applied to detect αfetoprotein, carcinoma antigen 125, carbohydrate antigen 153, and carcinoembryonic antigen and to screen patients with liver, breast, or ovarian cancers. This method showed wide linear ranges over 5 orders of magnitude and much lower detection limits than previously reported multiplexed immunoassays. The high throughput and acceptable stability, reproducibility, and accuracy showed good applicability of the proposed multiplex CL imaging immunoassay in clinical diagnosis. In conclusion, there is a great opportunity for introducing the adaptive chemiluminescence devices, since clinical diagnostics represents a huge, well-established and important analytical field. Structural requirements for chemiluminescent reactions and the different factors that affect the efficiency of analysis are included in the review. Chemiluminescence application in immunoassay is the new version for this review. Practical considerations are not included in the review since the main interest is to state, through the aforementioned applications, that chemiluminescence has been, is, and will be a versatile tool for pharmaceutical analysis in future years.
Authors and Affiliations
Dr. Anil Batta Dr. Anil Batta
Keywords
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