CITOTOXIC AND MUTAGEN ACTION OF SECONDARY METABOLITES PHOMOPSIS HELIANTHI M.
Journal Title: Наукові доповіді НУБіП України - Year 2018, Vol 6, Issue 6
Abstract
The purpose of study was studying effects of secondary metabolites Phomopsis helianthi on nuclear apparatus and plant cells division processes. The Phomopsis helianthi fungi accumulation results in nutrient agar of secondary metabolism are presented. It is determined that dynamics of phenol content in the culture liquid and its total antioxidant activity has lognormal dependence. The highest concentration of phenolic compounds is reached on the 17th day of cultivation (0.41 mg / ml). It was shown that the antioxidant activity of culture fluid is closely correlated with content of phenolic compounds (r = 0.96). After solid-phase extraction, small and nonpolar fungal synthesis products (v / v - 1/200) caused increased mitotic activity of Allium cepa test cultures. When extract concentration is doubled (v / v - 1/100) in test culture nuclear cells, DNA synthesis was violated, total or partial chromatin dispersion was observed, bridges were formed and picnotial nuclei and micronuclei appeared.
Authors and Affiliations
Ye. V. Syvoded, O. V. Kolesnichenko, А. F. Likhanov
Keywords
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- EP ID EP488673
- DOI 10.31548/dopovidi2018.06.003
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