Citral Protects Human Endothelial Cells Against Hydrogen Peroxide-induced Oxidative Stress

Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2020, Vol 17, Issue 5

Abstract

Objectives: Oxidative stress plays a major role in endothelial dysfunction. Citral is a monoterpene aldehyde with antioxidant properties. This study aimed to investigate the effect of citral on human umbilical vein endothelial cells (HUVECs) under hydrogen peroxide (H2O2)-induced oxidative stress. Materials and Methods: The cells were treated with citral (0.625-10 μg/mL) for 24 h before exposure to H2O2 (0.5 mM, 2 h). Cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The hydroperoxide concentrations and ferric reducing ability of plasma (FRAP) were measured in intra- and extracellular fluids. Results: Pretreatment of HUVECs with citral at concentrations of 5 and 10 μg/mL significantly enhanced the cell viability in H2O2-induced cytotoxicity. It reduced intracellular hydroperoxide levels at the concentrations of 5 and 10 μg/mL and extracellular hydroperoxide levels at the concentrations of 2.5-10 μg/mL. Pretreatment with citral significantly increased the FRAP value in intra- and extracellular fluids at the concentration range of 1.25-10 μg/mL. Conclusion: Antioxidant and cytoprotective effects were found for citral against oxidative damage induced by H2O2 in human endothelial cells. However, more studies in this area are needed to assess its clinical value for prevention and treatment of cardiovascular diseases.

Authors and Affiliations

Leila SAFAEIAN, Seyed Ebrahim SAJJADI, Hossein MONTAZERI, Farzaneh OHADI, Shaghayegh JAVANMARD

Keywords

Related Articles

Screening of Sacrificial Excipients for Arresting Devitrification of Itraconazole from Solid Dispersion

Objectives: The aim of the present investigation was to develop a solid dispersion of itraconazole (ITR) using sacrificial excipients like pregelatinized starch and spray-dried lactose alongside hydroxypropyl methylcellu...

Impact of Particle-Size Reduction on the Solubility and Antidiabetic Activity of Extracts of Leaves of Vinca rosea

Objectives: The present study aimed to enhance the aqueous solubility of methanol extract of leaves of Vinca rosea (family: Apocynaceae) by particle-size reduction using milling and to evaluate its antidiabetic activity....

An In Vitro Study on the Interactions of Pycnogenol® with Cisplatin in Human Cervical Cancer Cells

Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol® (PYC), a phenolic...

Novel Targets for Antimicrobials

Antimicrobial resistance (AMR) is the phenomenon developed by microorganism on exposure to antimicrobial agents, making them unresponsive. Development of microbial confrontation is a severe rising risk to global communit...

Investigation of Physiological Effects Induced by Dehydroepiandrosterone in Human Endothelial Cells and Ovarian Cancer Cell Line

Objectives: Dehydroepiandrosterone (DHEA) is an endogenous hormone that acts as a ligand for several cellular receptors. An age-dependent decline in circulating levels of DHEA is linked to changes in various physiologica...

Download PDF file
  • EP ID EP689999
  • DOI 10.4274/tjps.galenos.2019.71602
  • Views 144
  • Downloads 0

How To Cite

Leila SAFAEIAN, Seyed Ebrahim SAJJADI, Hossein MONTAZERI, Farzaneh OHADI, Shaghayegh JAVANMARD (2020). Citral Protects Human Endothelial Cells Against Hydrogen Peroxide-induced Oxidative Stress. Turkish Journal of Pharmaceutical Sciences, 17(5), -. https://europub.co.uk/articles/-A-689999