Close look in EMT, MAPK and inflammation: what differs in mouse embryonic stem, somatic and cancer cells?
Journal Title: Dokuz Eylul Universitesi Tip Fakultesi Dergisi - Year 2018, Vol 32, Issue 3
Abstract
INTRODUCTION: Life is a continuous cycle of the cells, composed of beginning, surviving and death. Cells keep dividing, differentiate and turn into somatic cells. The somatic cells at some point can give way to cancer cells and share characteristics of stem cells. The processes during these periods are important for the identifying the tendency to abnormality in somatic cells and transformation to the cancer cells. To control emergence and progress of the cancer, mechanisms take part in the surviving become significant, especially EMT and inflammation. EMT occurs in both embryonic developmental stages and cancer progression and molecular basis of this process can be the therapeutic target for cure METHODS: For cancer representation mouse squamous lung cancer cells (SqLCCs), for somatic origin mouse skin fibroblasts (MSFs) and for embryonic stem cell mouse embryonic stem cells (mESCs) were used for comparison of three signalling pathways (EMT, MAPK and inflammation) at the gene expression level. Immunofluorescence staining protein levels of ERK 1/2, Vimentin and Twist were compared RESULTS: ERK1/2 protein expression similar in MSFs and SqLCCs while mESCs expression wasthe lowest. Besides, Twist and Vimentin expression statistically different in three. According to gene expression profiling of the EMT and inflammation supported by the MAPK signalling pathway are a far cry from each other at prominent genes especially Sparc, Vimentin, Mapksp1 and Il24 DISCUSSION AND CONCLUSION: According to the results, three different cell linages showed different pattern both in gene and protein expression. Therefore, these moleculescan be the potential driving force for therapeutic target.
Authors and Affiliations
Fatih OLTULU, Berrin Özdil, Çevik Gürel, Eda Açıkgöz, duygu çalık kocatürk, Yasemin Adalı, Ayşegül UYSAL, Gülperi ÖKTEM, Hüseyin AKTUĞ
Keywords
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- EP ID EP440711
- DOI 10.5505/deutfd.2018.16768
- Views 79
- Downloads 0
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