Abstract

Background: This work was carried out in order to investigate the association between Interleukin-18 (IL-18) gene SNPs (+105A>C, "rs549908"; -137 G>C, "rs187238"; -607 C>A, "rs1946518"; and -656 G>T, "rs1946519") and RSA among Palestinian women residing in Gaza Strip. Methods: In this case-control study, samples from 200 women (100 suffering from RSA and 100 control) were examined. All participants were genotyped for IL-18 SNPs: -137 G>C, -607 C>A, +105A>C, and -656 G>T. Allele specific polymerase chain reaction (AS-PCR) was used to detect IL- 18 -137 G>C and -607 C>A SNPs whereas, restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping IL-18 +105A>C and -656 G>T polymorphisms. Results: The results revealed that there is no significant association between the allele/genotype frequencies of the investigated IL-18 SNPs and RSA in the study population. Though not significant, the two groups showed remarkable differences in terms of allele/genotype frequencies of SNP -137 G>C. Conclusion: Among the examined IL-18 SNPs the promoter -137 G>C may be important in RSA in the investigated population.

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