Abstract

A simple, rapid and accurate spectrophotometric method has been developed for estimation of metronidazole. The method involved measurement of absorbance at wavelength 320 nm using water as the solvent. The method was validated according to ICH guidelines with respect to different analytical parameters such as linearity, precision, accuracy, Limit of Detection (LOD) and Limit of Quantification (LOQ). Beer’s law obeyed in concentration range of 2-20 μg/ml. Limit of detection and quantification values were determined to be 0.763μg/ml and 2.312μg/ml respectively. The recovery values were found to be in the range of 98-102%. The proposed method was also found to be precise since %RSD value was less than 2 and can be used for routine quality control analysis.

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